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Aptamer based determination of the cancer biomarker HER2 by using phosphate-functionalized MnO2 nanosheets as the electrochemical probe

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Abstract

The authors report a sensitive electrochemical aptamer-based assay for the cancer biomarker human epidermal growth factor receptor-2 (HER2). It is based on the use of MnO2 nanosheets that were functionalized with phosphate and a HER2 binding aptamer and serve as the electrochemical probe. The assay follows a sandwich protocol. A peptide that can specifically recognize HER2 was immobilized on a gold electrode for the capture of HER2. Then, the functionalized MnO2 nanosheets were linked to the electrode via the binding between HER2 and HER2 aptamer on the MnO2 nanosheets. The reaction of phosphate and aptamer on the MnO2 nanosheets with molybdate leads to the formation of redox-active molybdophosphate. This results in dual signal amplification. The generated electrochemical current was measured at 0.22 V (vs. Ag/AgCl). The assay allows HER2 to be determined in the 0.1 to 500 pg·mL−1 concentration range, and the detection limit is as low as 0.05 pg·mL−1. The assay was successfully applied for the detection of HER2 in spiked human serum samples.

Electrochemical detection of breast cancer biomarker human epidermal growth factor receptor-2 (HER2) is reported utilizing phosphate ions and aptamer functionalized MnO2 nanosheet as probe.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (no. 21575165) and the Hunan Provincial Science and Technology Plan Project, China (no. 2016TP1007).

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Hunan Provincial Key Laboratory of Efficient and Clean Utilization of Manganese Resources, Central South University, Changsha, 410083, China

    Yuanlin Chai, Xiaoqing Li & Minghui Yang

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  1. Yuanlin Chai
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  2. Xiaoqing Li
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  3. Minghui Yang
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Correspondence to Minghui Yang.

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The authors declare that they have no competing interests.

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All experiments were in accordance with the guidelines of the National Institute of Health, China, and approved by the Institutional Ethical Committee (IEC) of Central South University. We also obtained informed consent for any experimentation with human serum samples.

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Chai, Y., Li, X. & Yang, M. Aptamer based determination of the cancer biomarker HER2 by using phosphate-functionalized MnO2 nanosheets as the electrochemical probe. Microchim Acta 186, 316 (2019). https://doi.org/10.1007/s00604-019-3412-y

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