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Fluorescent C-NanoDots for rapid detection of BRCA1, CFTR and MRP3 gene mutations

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The authors report on a fluorometric method for the rapid detection of BRCA1, CFRT and MRP3 gene mutations. These are associated with breast cancer, cystic fibrosis and autoimmune hepatitis diseases, respectively. Carbon nanodots with blue fluorescence (with excitation/emission maxima at 340/440 nm) were synthesized and characterized, and their interactions with DNA were investigated. Changes in the fluorescence intensity following interaction with ssDNA and dsDNA were used for specific DNA sequence of BRCA1, CFRT and MRP3 genes detection. The response to DNAs is linear up to 200 nM and the detection limit is 270 pM. The assay selectivity allows the detection of single gene mutations. Under optimum conditions, the assay can rapidly discriminate between wild type and mutated samples.

Schematic representation of fluorescence assay for rapid detection of gene mutation based on fluorescent carbon nanodots.

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CAM projects: TRANSNANOAVANSENS-CM (S2018/NMT-4349) and MAD2D-CM Program. MEIC projects: CTQ2017-84309-C2-1-R and MAT2015-71879-P. We thank the Confocal Microscopy and Flow Cytometry Services of CBMSO.

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Correspondence to Encarnación Lorenzo.

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García-Mendiola, T., Elosegui, C.G., Bravo, I. et al. Fluorescent C-NanoDots for rapid detection of BRCA1, CFTR and MRP3 gene mutations. Microchim Acta 186, 293 (2019).

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