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A single-shot diagnostic platform based on copper nanoclusters coated with cetyl trimethylammonium bromide for determination of carbamazepine in exhaled breath condensate

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Abstract

A fluorescent nanoprobe is designed for the determination of carbamazepine (CBZ) in exhaled breath condensate (EBC) of patients receiving CBZ. The probe consists of copper nanoclusters (Cu NCs) coated with cetyl trimethylammonium bromide. The interaction of probe with CBZ results in blocking non-radiative e−/h+ recombination defect sites on the surface of Cu NCs and consequently enhancing the blue-green fluorescence of Cu NCs (excitation/emission wavelengths: 290/480 nm). The experimental conditions were optimized using a response surface methodology (central composite design). Under the optimized conditions, the calibration plot is linear in the 0.2 to 20 μg mL−1 CBZ concentration range and the detection limit is as low as 0.08 μg mL−1. The intra-day and inter-day relative standard deviations for six replicated measurements of 10 μg mL−1 CBZ are 3.9% and 4.8%, respectively. The method was applied for the determination of CBZ level in EBC of patients receiving CBZ. The accuracy of the method was confirmed by HPLC-UV analysis as a reference method.

Schematic presentation of cetyl trimethylammonium bromide coated copper nanocluster's response to carbamazepine

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Acknowledgements

Research reported in this publication was supported by Elite Researcher Grant Committee under grant number 963572 from the National Institutes for Medical Research Development (NIMAD), Tehran, Iran.

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Authors and Affiliations

  1. Student Research Committee, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, 51664, Iran

    Ayda Hatefi

  2. Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, 51664, Iran

    Elaheh Rahimpour & Abolghasem Jouyban

  3. Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, 51664, Iran

    Maryam Khoubnasabjafari

  4. Neuroscience Research Center, Tabriz University of Medical Sciences, Tabriz, 51664, Iran

    Maryam Edalat

  5. Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, 51664, Iran

    Vahid Jouyban-Gharamaleki

  6. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, 51664, Iran

    Sima Alvani-Alamdari & Ali Nokhodchi

  7. Pharmaceutics Research Laboratory, Arundel Building, School of Life Sciences, University of Sussex, Brighton, BN1 9QG, UK

    Ali Nokhodchi

  8. Electroanalytical Chemistry Laboratory, Faculty of Chemistry, University of Tabriz, Tabriz, 5166616471, Iran

    Mohammad Hossein Pournaghi-Azar

  9. Kimia Idea Pardaz Azarbayjan (KIPA) Science Based Company, Tabriz University of Medical Sciences, Tabriz, 51664, Iran

    Abolghasem Jouyban

Authors
  1. Ayda Hatefi
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  2. Elaheh Rahimpour
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  3. Maryam Khoubnasabjafari
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  9. Abolghasem Jouyban
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Correspondence to Elaheh Rahimpour.

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Hatefi, A., Rahimpour, E., Khoubnasabjafari, M. et al. A single-shot diagnostic platform based on copper nanoclusters coated with cetyl trimethylammonium bromide for determination of carbamazepine in exhaled breath condensate. Microchim Acta 186, 194 (2019). https://doi.org/10.1007/s00604-019-3278-z

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  • DOI: https://doi.org/10.1007/s00604-019-3278-z

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