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Square-wave adsorptive anodic stripping voltammetric determination of norfloxacin using a glassy carbon electrode modified with carbon black and CdTe quantum dots in a chitosan film

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Abstract

A glassy carbon electrode was modified with carbon black and CdTe quantum dots in a chitosan film to obtained a sensor for norfloxacin (NOR) in the presence of dopamine, caffeine, and uric acid. The morphological, structural and electrochemical characteristics of the nanostructured material were evaluated using spectrophotometry, X-ray diffraction, transmission electronic microscopy and voltammetry. The high electrochemical activity, fast electron transfer rate and high surface area enhanced the oxidation peak currents and shifted the peak potentials of NOR for more negative values (typically at 0.95 V vs. Ag/AgCl). Electrochemical determination of NOR was carried out using square-wave adsorptive anodic stripping voltammetry (SWAdASV). Response is linear in the 0.2 to 7.4 μmol L−1 NOR concentration range, and the detection limit is as low as 6.6 nmol L−1. The method was successfully applied to the determination of norfloxacin in pharmaceutical formulation, synthetic urine and spiked serum.

Schematic presentation of a voltammetric method using a glassy carbon electrode modified with carbon black and CdTe quantum dots in a chitosan film for the determination of norfloxacin in serum and urine samples.

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Acknowledgements

The authors gratefully acknowledge the financial support granted by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) process number (160150/2015-9, 405546/2018-1 and 429462/2018-2) and FAPESP process number 2016/16565-5, 2016/12926-3 and 2017/10118-0.

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Authors and Affiliations

  1. Department of Chemistry, Federal University of São Carlos, Rod. Washington Luís km 235, P. O. Box 676, São Carlos, SP, 13560-970, Brazil

    Anderson Martin Santos, Ademar Wong, Fernando Cruz Moraes & Orlando Fatibello-Filho

  2. Institute of Chemistry, Centro de Tecnologia – Cidade Universitária, Federal University of Rio de Janeiro, Avenida Athos da Silveira Ramos, n° 149, Bloco A, Rio de Janeiro, RJ, Postal ZIP Code 21941-901, Brazil

    Fernando Henrique Cincotto

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  1. Anderson Martin Santos
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  2. Ademar Wong
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  3. Fernando Henrique Cincotto
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  5. Orlando Fatibello-Filho
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Correspondence to Fernando Cruz Moraes.

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Santos, A.M., Wong, A., Cincotto, F.H. et al. Square-wave adsorptive anodic stripping voltammetric determination of norfloxacin using a glassy carbon electrode modified with carbon black and CdTe quantum dots in a chitosan film. Microchim Acta 186, 148 (2019). https://doi.org/10.1007/s00604-019-3268-1

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