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Fluorometric determination of the activity of inorganic pyrophosphatase and its inhibitors by exploiting the peroxidase mimicking properties of a two-dimensional metal organic framework

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Abstract

A copper(II)-based two-dimensional metal-organic framework with nanosheet structure (CuBDC NS) that possesses peroxidase (POx) mimicking activity was prepared. In the presence of hydrogen peroxide, the system catalyses the oxidation of terephthalic acid to a blue-fluorescent product (excitation = 315 nm; emission = 425 nm). Pyrophosphate has a very strong affinity for Cu2+ ion and blocks the POx-mimicking activity of the CuBDC NS. If, however, inorganic pyrophosphatase is present, the POx mimicking activity is gradually restored because pyrophosphate is hydrolyzed. The findings were used to design a method for the determination of the activity of inorganic pyrophosphatase by fluorometry. Fluorescence increases linearly in the 1–50 mU·mL−1 inorganic pyrophosphatase activity range. The limit of detection is 0.6 mU·mL−1 (S/N = 3).

A copper(II)-based two-dimensional metal-organic framework (CuBDC NS) is described that possesses POx-mimicking activity. Inorganic pyrophosphate (PPi) was hydrolyzed to phosphate in the presence of inorganic pyrophosphatase (PPase). Hence, it cannot coordinate with Cu2+ in CuBDC NS, its structure was well-conserved to catalyses the oxidation of terephthalic acid (H2BDC) to produce a blue fluorescent product (oxBDC) in the presence of hydrogen peroxide (H2O2).

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Acknowledgments

The authors gratefully acknowledged the financial support of the National Natural Science Foundation of China (21375021), STS Key Project of Fujian Province (2017 T3007), Nature Sciences Funding of Fujian Province (2018 J01682) and the Project of Fuzhou Science and Technology Bureau (2016-G-66), Key Project of Fujian Province (2015Y0050).

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Authors and Affiliations

  1. Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian, 350108, People’s Republic of China

    Shuisheng Hu, Lin Zhu, Longhua Guo, Zhenyu Lin, Bin Qiu & Guonan Chen

  2. Department of Applied Biology and Chemical Technology and State Key Laboratory of Chemical Biology and Drug Discovery, The Hong Kong Polytechnic University, Hunghom, Kowloon, Hong Kong, People’s Republic of China

    Shuisheng Hu, Cheong Wing Lam & Kwok Yin Wong

  3. Department of Medical Oncology, Fujian Provincial Clinical College Affiliated with Fujian Medical University, Fujian Provincial Hospital, Fuzhou, 350001, People’s Republic of China

    Zhenhua Liu

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  1. Shuisheng Hu
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  2. Lin Zhu
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  4. Longhua Guo
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Correspondence to Bin Qiu or Zhenhua Liu.

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Hu, S., Zhu, L., Lam, C.W. et al. Fluorometric determination of the activity of inorganic pyrophosphatase and its inhibitors by exploiting the peroxidase mimicking properties of a two-dimensional metal organic framework. Microchim Acta 186, 190 (2019). https://doi.org/10.1007/s00604-019-3250-y

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