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Multifunctional DNA nanocage with CdTe quantum dots for fluorescence detection of human 8-oxoG DNA glycosylase 1 and doxorubicin delivery to cancer cells

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Abstract

A multifunctional DNA nanocage containing CdTe quantum dots (QDs) was prepared. It was applied to the fluorometric detection of human 8-oxoG DNA glycosylase 1 (hOGG1) by exonuclease-assisted cycling amplification technique. When loaded with the cancer drug doxorubicin (Dox), the nanocage is also a versatile probe for fluorescence imaging of cancer cells, and drug delivery to them. The presence of hOGG1 leads to the division of DNA HP1 (containing 8-oxo-dG) and formation of DNA fragments 1 and 2. Then, HP2 is added to hybridize with DNA 1 and produced lots of trigger DNA (containing nucleolin aptamer) by Exo III-aided cycling amplification. The DNA nanocage was fabricated by linking the trigger DNA to multiple specific DNA strands, and the fluorescent CdTe QDs were further conjugated to the DNA nanocage for sensitive detection of hOGG1 activity. After Dox is incorporated into the DNA nanocage, the fluorescence of Dox is turned off. Once the DNA nanocage enters the MCF-7 cells, the Dox is released and its fluorescence (measured at excitation/emission wavelengths of 480/560 nm) is turned on. The DNA nanocage containing fluorescent QDs and Dox was successfully applied to the fluorometric detection of hOGG1, fluorescence imaging, and therapy of cancer cells, which has great promise in clinical application and treatment of cancer.

A multifunctional DNA nanocage containing CdTe quantum dots and acting as a signalling probe was prepared. It was applied to fluorometric determination of human 8-oxoG DNA glycosylase 1 using cycling amplification technique. It also enables drug delivery to cancer cells if loaded with doxorubicin.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No.21575072), and the Key Research and Development Plan of Shandong Province (2016GGX102021).

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Authors and Affiliations

  1. Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, Qingdao University of Science and Technology, Qingdao, 266042, People’s Republic of China

    Guifen Jie, Xiaoshan Gao, Junjun Ge & Chunli Li

  2. Shandong Key Laboratory of Biochemical Analysis, Qingdao University of Science and Technology, Qingdao, 266042, People’s Republic of China

    Guifen Jie, Xiaoshan Gao, Junjun Ge & Chunli Li

  3. Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong, Qingdao University of Science and Technology, Qingdao, 266042, People’s Republic of China

    Guifen Jie, Xiaoshan Gao, Junjun Ge & Chunli Li

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  1. Guifen Jie
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  2. Xiaoshan Gao
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Correspondence to Guifen Jie.

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Jie, G., Gao, X., Ge, J. et al. Multifunctional DNA nanocage with CdTe quantum dots for fluorescence detection of human 8-oxoG DNA glycosylase 1 and doxorubicin delivery to cancer cells. Microchim Acta 186, 85 (2019). https://doi.org/10.1007/s00604-018-3199-2

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  • DOI: https://doi.org/10.1007/s00604-018-3199-2

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