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A turn-on fluorescent probe for vitamin C based on the use of a silicon/CoOOH nanoparticle system

Abstract

The authors describe a fluorometric method for the turn-on determination of vitamin C (ascorbic acid). The blue fluorescence of silicon nanoparticles (SiNPs; with excitation/emission maxima at 350/450 nm) is found to be quenched by CoOOH nanoparticles (NPs). In the presence of vitamin C, the CoOOH NPs are decomposed by a redox reaction between the diol group of vitamin C and CoOOH NPs. As a result, fluorescence recovers. On the basis of this finding, a fluorometric method was designed for the turn-on detection of vitamin C. Under optimal conditions, the method has a low detection limit (0.47 μM) and a linear response in the 0.5 μM to 20 μM a concentration range. It was successfully applied to the determination of vitamin C in spiked red grape and orange juice, and in vitamin C tablets.

A target-triggered dissociation of quencher-based strategy for the fluorescence “turn-on” detection of vitamin C was developed. It is based on surface energy transfer (SET) and an inner filter effect (IFE) between silicon nanoparticles and CoOOH nanoparticles as well as the redox reaction between vitamin C and CoOOH nanoparticles.

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References

  1. Carr A, Maggini S (2017) Vitamin C and immune function. Nutrients 9(11):1211

    Article  Google Scholar 

  2. Frei B, England L, Ames BN (1989) Ascorbate is an outstanding antioxidant in human blood plasma. P Natl Acad Sci 86(16):6377–6381

    CAS  Article  Google Scholar 

  3. Fritz H, Flower G, Weeks L, Cooley K, Callachan M, McGowan J, Skidmore B, Kirchner L, Seely D (2014) Intravenous vitamin C and cancer:a systematic review. Integr Cancer Ther 13(4):280–300

    CAS  Article  Google Scholar 

  4. Lane DJR, Richardson DR (2014) The active role of vitamin C in mammalian iron metabolism: much more than just enhanced iron absorption! Free Radical Bio Med 75:69–83

    CAS  Article  Google Scholar 

  5. Linster CL, Van Schaftingen E (2007) Vitamin C. FEBS J 274(1):1–22

    CAS  Article  Google Scholar 

  6. Carpenter KJ (2012) The discovery of vitamin C. Ann Nutr Metab 61(3):259–264

    CAS  Article  Google Scholar 

  7. Spínola V, Llorent-Martínez EJ, Castilho PC (2014) Determination of vitamin C in foods: current state of method validation. J Chromatogr A 1369:2–17

    Article  Google Scholar 

  8. Maduraiveeran G, Sasidharan M, Ganesan V (2018) Electrochemical sensor and biosensor platforms based on advanced nanomaterials for biological and biomedical applications. Biosens Bioelectron 103:113–129

    CAS  Article  Google Scholar 

  9. Yang P, Gao X, Wang L, Wu Q, Chen Z, Lin X (2014) Amperometric sensor for ascorbic acid based on a glassy carbon electrode modified with gold-silver bimetallic nanotubes in a chitosan matrix. Microchim Acta 181(1):231–238

    CAS  Article  Google Scholar 

  10. Huang W, Deng Y, He Y (2017) Visual colorimetric sensor array for discrimination of antioxidants in serum using MnO2 nanosheets triggered multicolor chromogenic system. Biosens Bioelectron 91:89–94

    CAS  Article  Google Scholar 

  11. Hashmi MH, Adil AS, Viegas A, Ahmad I (1970) Microdetermination of ascorbic acid and tryptophan by colorimetry. Microchim Acta 58(3):457–462

    Article  Google Scholar 

  12. Liu S, Pang S (2018) A dual-model strategy for fluorometric determination of ascorbic acid and of ascorbic acid oxidase activity by using DNA-templated gold-silver nanoclusters. Microchim Acta 185(9):426

    Article  Google Scholar 

  13. Liu X, Na W, Liu H, Su X (2017) Fluorescence turn-off-on probe based on polypyrrole/graphene quantum composites for selective and sensitive detection of paracetamol and ascorbic acid. Biosens Bioelectron 98:222–226

    CAS  Article  Google Scholar 

  14. Chen X, Lu Q, Liu D, Wu C, Liu M, Li H, Zhang Y, Yao S (2018) Highly sensitive and selective determination of copper(II) based on a dual catalytic effect and by using silicon nanoparticles as a fluorescent probe. Microchim Acta 185(3):188

    Article  Google Scholar 

  15. Xu H, Gu B, Li Y, Huang Z, Su W, Duan X, Yin P, Li H, Yao S (2018) A highly selective, colorimetric and ratiometric fluorescent probe for NH2NH2 and its bioimaging. Talanta 180:199–205

    CAS  Article  Google Scholar 

  16. Liu Q, Lai Q, Li N, Su X (2018) Copper nanoclusters capped with tannic acid as a fluorescent probe for real-time determination of the activity of pyrophosphatase. Microchim Acta 185(3):182

    Article  Google Scholar 

  17. Na W, Hu T, Su X (2017) Turn-on fluorometric NADPH assay using orange emitting graphene oxide quantum dots. Microchim Acta 184(12):4571–4578

    CAS  Article  Google Scholar 

  18. Wang Y, Lu L, Peng H, Xu J, Wang F, Qi R, Xu Z, Zhang W (2016) Multi-doped carbon dots with ratiometric pH sensing properties for monitoring enzyme catalytic reactions. Chem Commun 52(59):9247–9250

    CAS  Article  Google Scholar 

  19. Jiang C, Shen Z, Luo C, Lin H, Huang R, Wang Y, Peng H (2016) One-pot aqueous synthesis of gadolinium doped CdTe quantum dots with dual imaging modalities. Talanta 155:14–20

    CAS  Article  Google Scholar 

  20. Li Q, Peng H, Wang J, Wang Y, Guo F (2015) Coexpression of CdSe and CdSe/CdS quantum dots in live cells using molecular hyperspectral imaging technology. J Biomed Opt 20(11):110504

    Article  Google Scholar 

  21. Lu Q, Zhao J, Xue S, Yin P, Zhang Y, Yao S (2015) A “turn-on” fluorescent sensor for ultrasensitive detection of melamine based on a new fluorescence probe and AuNPs. Analyst 140(4):1155–1160

    CAS  Article  Google Scholar 

  22. Xiao T, Sun J, Zhao J, Wang S, Liu G, Yang X (2018) FRET effect between fluorescent polydopamine nanoparticles and MnO2 nanosheets and its application for sensitive sensing of alkaline phosphatase. ACS Appl Mater Interfaces 10(7):6560–6569

    CAS  Article  Google Scholar 

  23. Zhao W, Brook MA, Li Y (2008) Design of gold nanoparticle-based colorimetric biosensing assays. ChemBioChem 9(15):2363–2371

    CAS  Article  Google Scholar 

  24. Feng Y, Liu Y, Su C, Ji X, He Z (2014) New fluorescent pH sensor based on label-free silicon nanodots. Sensors Actuators B Chem 203:795–801

    CAS  Article  Google Scholar 

  25. Wu QD, Gao XP, Li GR, Pan GL, Yan TY, Zhu HY (2007) Microstructure and electrochemical properties of Al-substituted nickel hydroxides modified with CoOOH nanoparticles. J Phys Chem C 111(45):17082–17087

    CAS  Article  Google Scholar 

  26. Wang J, Peng X, Li D, Jiang X, Pan Z, Chen A, Huang L, Hu J (2017) Ratiometric ultrasensitive fluorometric detection of ascorbic acid using a dually emitting CdSe@SiO2@CdTe quantum dot hybrid. Microchim Acta 185(1):42

    Article  Google Scholar 

  27. Mo Q, Liu F, Gao J, Zhao M, Shao N (2018) Fluorescent sensing of ascorbic acid based on iodine induced oxidative etching and aggregation of lysozyme-templated silver nanoclusters. Anal Chim Acta 1003:49–55

    CAS  Article  Google Scholar 

  28. Meng H, Yang D, Tu Y, Yan J (2017) Turn-on fluorescence detection of ascorbic acid with gold nanolcusters. Talanta 165:346–350

    CAS  Article  Google Scholar 

  29. Zhu L, Peng X, Li H, Zhang Y, Yao S (2017) On–off–on fluorescent silicon nanoparticles for recognition of chromium(VI) and hydrogen sulfide based on the inner filter effect. Sensors Actuators B Chem 238:196–203

    CAS  Article  Google Scholar 

  30. Lu Q, Chen X, Liu D, Wu C, Liu M, Li H, Zhang Y, Yao S (2018) Synergistic electron transfer effect-based signal amplification strategy for the ultrasensitive detection of dopamine. Talanta 182:428–432

    CAS  Article  Google Scholar 

  31. Braun G, Pavel I, Morrill AR, Seferos DS, Bazan GC, Reich NO, Moskovits M (2007) Chemically patterned microspheres for controlled nanoparticle assembly in the construction of SERS hot spots. J Am Chem Soc 129(25):7760–7761

    CAS  Article  Google Scholar 

  32. Braun GB, Lee SJ, Laurence T, Fera N, Fabris L, Bazan GC, Moskovits M, Reich NO (2009) Generalized approach to SERS-active nanomaterials via controlled nanoparticle linking, polymer encapsulation, and small-molecule infusion. J Phys Chem C 113(31):13622–13629

    CAS  Article  Google Scholar 

  33. Chao M-R, Hu C-W, Chen J-L (2016) Fluorometric determination of copper(II) using CdTe quantum dots coated with 1-(2-thiazolylazo)-2-naphthol and an ionic liquid. Microchim Acta 183(4):1323–1332

    CAS  Article  Google Scholar 

  34. Lu Q, Deng J, Hou Y, Wang H, Li H, Zhang Y, Yao S (2015) Hydroxyl-rich C-dots synthesized by a one-pot method and their application in the preparation of noble metal nanoparticles. Chem Commun 51(33):7164–7167

    CAS  Article  Google Scholar 

  35. Zu F, Yan F, Bai Z, Xu J, Wang Y, Huang Y, Zhou X (2017) The quenching of the fluorescence of carbon dots: a review on mechanisms and applications. Microchim Acta 184(7):1899–1914

    CAS  Article  Google Scholar 

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (21475043, 21874042, and 21675051), Foundation of the Science & Technology Department of Hunan Province (2016SK2020), and Project funded by China Postdoctoral Science Foundation (2018 M640753).

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Correspondence to Youyu Zhang.

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Research Highlights

• A label free target-triggered dissociation of quencher-based strategy for vitamin C was designed based on vitamin C-triggered destruction of CoOOH nanoparticles

• This target-triggered dissociation of CoOOH nanoparticles-based strategy was high sensitivity

• This method has been successfully applied to the detection of vitamin C in spiked food, beverage and tablet samples with satisfactory results

• This target-triggered dissociation of quencher-based strategy will enrich the design strategies of biosensors

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Lu, Q., Chen, X., Liu, D. et al. A turn-on fluorescent probe for vitamin C based on the use of a silicon/CoOOH nanoparticle system. Microchim Acta 186, 72 (2019). https://doi.org/10.1007/s00604-018-3181-z

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  • DOI: https://doi.org/10.1007/s00604-018-3181-z

Keywords

  • Quenching
  • Redox reaction
  • Surface energy transfer
  • Inner filter effect
  • Silicon nanoparticles
  • Fluorometry
  • Stern-Volmer plot
  • Fluorescence “turn-on” strategy
  • Cobalt oxyhydroxide nanoparticles