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A glassy carbon electrode with electrodeposited silver nanoparticles for aptamer based voltammetric determination of trinitrotoluene using riboflavin as a redox probe

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Abstract

An electrochemical nanoaptasensor is described that is based on the use of a glassy carbon electrode (GCE) modified with electrodeposited silver nanoparticles (AgNPs). An aptamer (Apt) against trinitrotoluene (TNT) was then immobilized on the AgNPs. The addition of TNT to the modified GCE leads to decrease in peak current (typically measured at a potential of −0.45 V vs. Ag/AgCl) of riboflavin which acts as an electrochemical probe. Even small changes in the surface (as induced by binding of Apt to TNT) alter the interfacial properties. As a result, the LOD is lowered to 33 aM, and the dynamic range extends from 0.1 fM to 10 μM without sacrificing specificity.

Schematic presentation of a nanoaptasensor which is based on a glassy carbon electrode (GCE) modified with electrodeposited silver nanoparticles (AgNPs) and aptamer (Apt). It was applied to the detection of 2,4,6-trinitrotoluene (TNT) with the help of riboflavin (RF) as a redox probe.

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Acknowledgements

This work was funded by Ilam University and the financial support of this University is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Chemistry, Ilam University, PO. Box 69315-516, Ilam, Iran

    Mahmoud Roushani & Faezeh Shahdost-fard

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  1. Mahmoud Roushani
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  2. Faezeh Shahdost-fard
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Correspondence to Mahmoud Roushani or Faezeh Shahdost-fard.

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Roushani, M., Shahdost-fard, F. A glassy carbon electrode with electrodeposited silver nanoparticles for aptamer based voltammetric determination of trinitrotoluene using riboflavin as a redox probe. Microchim Acta 185, 558 (2018). https://doi.org/10.1007/s00604-018-3098-6

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  • DOI: https://doi.org/10.1007/s00604-018-3098-6

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