Abstract
A sorbent for selective extraction of phosphoproteins was obtained by immobilization of a Ce(IV)-substituted polyoxometalate on ethylenediamine-functionalized graphene oxide (CeEGO). The resulting composites exhibit an adsorption capacity of 981 mg g−1 for β-casein due to the synergistic effect of metal-affinity interaction between Ce(IV) and phosphate groups and π-stacking interaction between the polyoxometalate framework and the phosphate groups. The results of LC-MS and SDS-PAGE analysis show that the CeEGO composites can be applied to the extraction of phosphoproteins from protein mixture, and as little as 50 μg mL−1 of the phosphoprotein β-casein can be detected by SDS-PAGE. It was also applied to the extraction of β-casein from spiked biological samples such as drinking milk, whole blood and swine heart tissue extract.
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Acknowledgments
This work is financially supported by the National Natural Science Foundation of China (21605014, 21475018 and 21727811) and the Fundamental Research Funds for the Central Universities (N160504010).
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Wang, MM., Chen, S., Zhang, DD. et al. Immobilization of a Ce(IV)-substituted polyoxometalate on ethylenediamine-functionalized graphene oxide for selective extraction of phosphoproteins. Microchim Acta 185, 553 (2018). https://doi.org/10.1007/s00604-018-3095-9
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DOI: https://doi.org/10.1007/s00604-018-3095-9