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A fluorometric assay for staphylococcal enterotoxin B by making use of platinum coated gold nanorods and of upconversion nanoparticles

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Abstract

An aptamer based fluorometric assay is presented for fast and accurate detection of staphylococcal enterotoxin B (SEB). It is making use of platinum-coated gold nanorods (AuNR@Pt) and upconversion nanoparticles (UCNPs). The aptamer against SEB is immobilized on AuNR@Pt while the complementary DNA fragment of SEB aptamer is immobilized on UCNPs. As the concentration of SEB increases, the fluorescence of the satellite assembly (AuNR@Pt-UCNPs) is gradually restored. Under the optimized conditions, fluorescence (best measured at excitation/emission wavelengths of 980/543 nm) linearly increases in the 2.0–400 pg·mL−1 SEB concentration range. The limit of detection is as low as 0.9 pg·mL−1 (at an S/N of 3), significantly lower than existing methods. The method was applied to the determination of SEB in spiked milk samples. The average recoveries ranged from 91.2% to 104.6%, confirming the practicality of this method.

Schematic illustration of a fluorometric assay based on inner filter effect (IFE) between platinum coated gold nanorods (AuNR@Pt) and upconversion nanoparticles (UCNPs) for the determination of staphylococcal enterotoxin B (SEB).

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Acknowledgements

This research was supported by Nature Science Foundation of Jiangsu Province (No. BK20160168), Nature Science Foundation of China (Nos. 31601413 and 31501418) and Special Funds for Taishan Scholars Project.

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Author notes

  1. Zhengzong Wu and Deyun He contributed equally to the work and should be regarded as co-first authors

Authors and Affiliations

  1. State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, China

    Zhengzong Wu & Bo Cui

  2. State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100094, China

    Deyun He

Authors
  1. Zhengzong Wu
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  2. Deyun He
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  3. Bo Cui
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Correspondence to Zhengzong Wu or Bo Cui.

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Wu, Z., He, D. & Cui, B. A fluorometric assay for staphylococcal enterotoxin B by making use of platinum coated gold nanorods and of upconversion nanoparticles. Microchim Acta 185, 516 (2018). https://doi.org/10.1007/s00604-018-3058-1

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  • DOI: https://doi.org/10.1007/s00604-018-3058-1

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