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Fluorometric determination of quercetin by using graphitic carbon nitride nanoparticles modified with a molecularly imprinted polymer

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Abstract

The authors describe a fluorescent probe for sensitive and selective determination of quercetin, an indicator for the freshness of drinks. The probe consists of silica ball encapsulated graphitic carbon nitride (g-C3N4) modified with a molecularly imprinted polymer (MIP). It was synthesized via reverse microemulsion. The resulting MIP@g-C3N4 nanocomposite was characterized by fluorescence spectroscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, and X-ray powder diffraction. Quercetin quenches the fluorescence of the MIP@g-C3N4 probe. The effect was used to quantify quercetin in grape juice, tea juice, black tea, and red wine by fluorometry (λexc = 350 nm, λem = 460 nm). Response is linear in the 10–1000 ng mL−1 quercetin concentration range. The detection limit is 2.5 ng mL−1, recoveries range between 90.7 and 94.1%, and relative standard deviations are between 2.1 and 5.5%.

Schematic of the synthesis of the MIP@g-C3N4 by a reverse microemulsion method. The probe was applied for the selective recognition and fluorometric determination of quercetin.

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Acknowledgments

This project was funded by the Fundamental Research Funds for the Central Universities (No. 2572017 EB08), Harbin science and technology innovation talent research special funds (2016RAQXJ151) and Natural Science Foundation of Heilongjiang Province (JJ2018ZR0081).

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Authors and Affiliations

  1. Department of Chemistry, College of Science, Northeast Forestry University, 26 Hexing Road, Harbin, 150040, People’s Republic of China

    Shengnan Xu & Ligang Chen

  2. College of Forestry, Northeast Forestry University, 26 Hexing Road, Harbin, 150040, People’s Republic of China

    Ling Ma

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  1. Shengnan Xu
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  2. Ligang Chen
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Correspondence to Ligang Chen or Ling Ma.

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Xu, S., Chen, L. & Ma, L. Fluorometric determination of quercetin by using graphitic carbon nitride nanoparticles modified with a molecularly imprinted polymer. Microchim Acta 185, 492 (2018). https://doi.org/10.1007/s00604-018-3016-y

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