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Graphene oxide wrapped with gold nanorods as a tag in a SERS based immunoassay for the hepatitis B surface antigen

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Abstract

A composite consisting of graphene oxide and gold nanorods (GO-GNRs) was designed for the trace determination of hepatitis B surface antigen (HBsAg) using surface enhanced Raman spectroscopy (SERS). GO contains numerous carboxy and hydroxy groups on its surface and therefore can serve as the substrate for decoration with GNRs and for immobilizing antibody against HBsAg. The GNRs (carrying the SERS probe 2-mercaptopyridine) exhibit high SERS activity, and this improves the sensitivity of the biosensor. The antibody on the GO-GNRs binds HBsAg with high specificity, and it results in excellent selectivity. The SERS signal (measured at 1002 cm−1) increases in the 1–1000 pg·mL−1 HBsAg concentrations range, and the limit of detection is 0.05 pg·mL−1 (at an S/N ratio of 3). The immunoassay achieves the sensitive and selective determination of HBsAg in serum and expands the potential application of GO-GNR based SERS tag in clinical research.

A novel graphene oxide-gold nanorod (GO-GNRs) based surface-enhanced Raman scattering (SERS) tag for immunoassay was designed. It allows for sensitive and selective determination of HBsAg in serum. The method is expected to expand the potential application in the environment, in medicine and in food analysis.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (81472001, 31400851), the Minjiang Scholars Program of Fujian Province, the Tongjiang Scholars Program of Quanzhou City, the Fourth Health Education Joint Development Project of Fujian Province (WKJ-2016-2-36). Natural Science Foundation of Fujian Province (2015 J05030).

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Author notes

  1. Minghuan Liu and Chaohui Zheng contributed equally to this work.

Authors and Affiliations

  1. The Second Affiliated Hospital of Fujian Medical University, Quanzhou, 362000, Fujian Province, China

    Minghuan Liu, Chaohui Zheng & Da-Peng Yang

  2. College of Chemical Engineering and Materials Science, Quanzhou Normal University, Fujian Province, China

    Minghuan Liu, Malin Cui, Xiaoyan Zhang & Da-Peng Yang

  3. Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China

    Xiansong Wang

  4. Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent Instrument for Diagnosis and Therapy, Department of Instrument Science & Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China

    Daxiang Cui

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  1. Minghuan Liu
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  2. Chaohui Zheng
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  3. Malin Cui
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  4. Xiaoyan Zhang
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  5. Da-Peng Yang
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Correspondence to Da-Peng Yang, Xiansong Wang or Daxiang Cui.

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Liu, M., Zheng, C., Cui, M. et al. Graphene oxide wrapped with gold nanorods as a tag in a SERS based immunoassay for the hepatitis B surface antigen. Microchim Acta 185, 458 (2018). https://doi.org/10.1007/s00604-018-2989-x

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  • DOI: https://doi.org/10.1007/s00604-018-2989-x

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