An electrochemical switching strategy is presented for the sensitive determination of Staphylococcus enterotoxin B (SEB). It is based on the use of DNA triangular pyramid frustum nanostructure (TPFDNA) consisting of (a) three thiolated probes, (b) one auxiliary probe, and (c) an aptamer against SEB. The TPFDNA was assembled on the gold electrode, with the SEB aptamer designed on top of the TPFDNA. The electron transfer to hexacyanoferrate acting as an electrochemical probe is strongly inhibited in the TPFDNA-modified electrode. This is assumed to be due to the formation of a 3D TPFDNA structure that limits access of hexacyanoferrate to the electrode. Therefore, the Faradaic impedance is large. However, in the presence of SEB, it will bind to the aptamer and dehybridize the hybrid formed between aptamer and its complementary sequence. As a result, the TPFDNA nanostructure changes to an equilateral triangle DNA nanostructure. This results in a more efficient electron transfer and a smaller Faradaic impedance. The method has a detection limit of 0.17 ng mL−1 of SEB (at an S/N of 3) and a dynamic range that covers the 0.2–1000 ng mL−1 concentration range. The applicability and reliability of the method was demonstrated by anayzing (spiked) milk samples, and the results were compared to those obtained with an ELISA kit. The relative standard deviations between the two methods range between −6.59 and 9.33%.
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Pinchuk IV, Beswick EJ, Reyes VE (2010) Staphylococcal enterotoxins. Toxins 2:2177–2197. https://doi.org/10.3390/toxins2082177
Wang HW, Wang HH, Liang LJ, Xu XL, Zhou GH (2018) Prevalence, genetic characterization and biofilm formation in vitro of Staphylococcus aureus isolated from raw chicken meat at retail level in Nanjing, China. Food Control 86:11–18. https://doi.org/10.1016/j.foodcont.2017.10.028
Nunes MM, Caldas ED (2017) Preliminary quantitative microbial risk assessment for Staphylococcus enterotoxins in fresh minas cheese, a popular food in Brazil. Food Control 73:524–531. https://doi.org/10.1016/j.foodcont.2016.08.046
Fisher EL, Otto M, Cheung GYC (2018) Basis of virulence in enterotoxin-mediated staphylococcal food poisoning. Front Microbiol 9(436). https://doi.org/10.3389/fmicb.2018.00436
Wang WB, Wang WW, Liu LQ, Xu LG, Kuang H, Zhu JP, Xu CL (2016) Nanoshell-enhanced Raman spectroscopy on a microplate for staphylococcal enterotoxin B sensing. ACS Appl Mater Interfaces 8:15591–15597. https://doi.org/10.1021/acsami.6b02905
Sorensen M, Klingenberg C, Wickman M, Sollid JUE, Furberg AS, Bachert C, Bousquet J (2017) Staphylococcus aureus enterotoxin sensitization is associated with allergic poly-sensitization and allergic multimorbidity in adolescents. Allergy 72:1548–1555. https://doi.org/10.1111/all.13175
Singh PK, Agrawal R, Kamboj DV, Gupta G, Boopathi M, Goel AK, Singh L (2010) Construction of a single-chain variable-fragment antibody against the superantigen staphylococcal enterotoxin B. Appl Environ Microbiol 76:8184–8191. https://doi.org/10.1128/AEM.01441-10
Deng RN, Wang L, Yi GZ, Hua EH, Xie GM (2014) Target-induced aptamer release strategy based on electrochemical detection of staphylococcal enterotoxin B using GNPs-ZrO2-chits film. Colloid Surf B 120:1–7. https://doi.org/10.1016/j.colsurfb.2014.04.028
Wu SJ, Duan N, Gu HJ, Hao LL, Ye H, Gong WH, Wang ZP (2016) A review of the methods for detection of Staphylococcus aureus enterotoxins. Toxins 8:176. https://doi.org/10.3390/toxins8070176
Rodriguez A, Gordillo R, Andrade MJ, Cordoba JJ, Rodriguez M (2016) Development of an efficient real-time PCR assay to quantify enterotoxin-producing staphylococci in meat products. Food Control 60:302–308. https://doi.org/10.1016/j.foodcont.2015.07.040
Ludwig S, Jimenez-Bush I, Brigham E, Bose S, Diette G, McCormack MC, Matsui EC, Davis MF (2017) Analysis of home dust for Staphylococcus aureus and staphylococcal enterotoxin genes using quantitative PCR. Sci Total Environ 581:750–755. https://doi.org/10.1016/j.scitotenv.2017.01.003
Andjelkovic M, Tsilia V, Rajkovic A, De Cremer K, VanLoco J (2016) Application of LC-MS/MS MRM to determine staphylococcal enterotoxins (SEB and SEA) in milk. Toxins 8:118. https://doi.org/10.3390/toxins8040118
Mondal B, Bhavanashri N, Ramial S, Kingston J (2018) Colorimetric DNAzyme biosensor for convenience detection of enterotoxin B harboring Staphylococcus aureus from food samples. J Agric Food Chem 66:1516–1522. https://doi.org/10.1021/acs.jfc.7b04820
Wang XL, Huang YK, Wu SJ, Duan N, Xu BC, Wang ZP (2016) Simultaneous detection of Staphylococcus aureus and Salmonella typhimurium using multicolor time-resolved fluorescence nanoparticles as labels. Int J Food Microbiol 237:172–179. https://doi.org/10.1016/j.ijfoodmicro.2016.08.028
Wu LY, Gao B, Zhang F, Sun XL, Zhang YZ, Li ZJ (2013) A novel electrochemical immunosensor based on magnetosomes for detection of staphylococcal enterotoxin B in milk. Talanta 106:360–366. https://doi.org/10.1016/j.talanta.2012.12.053
Seshadri P, Manoli K, Schneiderhan-Marra N, Anthes U, Wierzchowiec P, Bonrad K, DiFranco CD, Torsi L (2018) Low-picomolar, label-free procalcitonin analytical detection with an electrolyte-gated organic field-effect transistor based electronic immunosensor. Biosens Bioelectron 104:113–119. https://doi.org/10.1016/j.bios.2017.12.041
Abnous K, Danesh NM, Alibolandi M, Ramezani M, Emrani AS, Zolfaghari R, Taghdisi SM (2017) A new amplified π-shape electrochemical aptasensor for ultrasensitive detection of aflatoxin B1. Biosens Bioelectron 94:374–379. https://doi.org/10.1016/j.bios.2017.03.028
Zheng WL, Teng J, Cheng L, Ye YW, Pan DD, Wu JJ, Xue F, Liu GD, Chen W (2016) Hetero-enzyme-based two-round signal amplification strategy for trace detection of aflatoxin B1 using an electrochemical aptasensor. Biosens Bioelectron 80:574–581. https://doi.org/10.1016/j.bios.2016.01.091
Nguyen VT, Kwon YS, Gu MB (2017) Aptamer-based environmental biosensors for small molecule contaminants. Curr Opin Biotechnol 45:15–23. https://doi.org/10.1016/j.copbio.2016.11.020
Mondal B, Ramlal S, Lavu PS, Bhavanashri N, Kingston J (2018) Highly sensitive colorimetric biosensor for staphylococcal enterotoxin B by a label-free aptamer and gold nanoparticles. Front Microbiol 9(179). https://doi.org/10.3389/fmicb.2018.00179
Hasanzadeh M, Shadjou N, delaGuardia M (2017) Aptamer-based assay of biomolecules: recent advances in electro-analytical approach. Trac-Trend Anal Chem 89:119–132. https://doi.org/10.1016/j.trac.2017.02.003
Zeng Y, Zhang D, Qi P, Zheng LB (2017) Colorimetric detection of DNA by using target catalyzed DNA nanostructure assembly and unmodified gold nanoparticles. Microchim Acta 184:4809–4815. https://doi.org/10.1007/s00604-017-2463-1
Huang RR, He NY, Li ZY (2018) Recent progresses in DNA nanostructure-based biosensors for detection of tumor markers. Biosens Bioelectron 109:27–34. https://doi.org/10.1016/j.bios.2018.02.053
Taghdisi SM, Danesh NM, Ramezani M, Emrani AS, Abnous K (2016) A novel electrochemical aptasensor based on Y-shape structure of dual-aptamer-complementary strand conjugate for ultrasensitive detection of myoglobin. Biosens Bioelectron 80:532–537. https://doi.org/10.1016/j.bios.2016.02.029
Sheng QL, Liu RX, Zhang S, Zheng JB (2014) Ultrasensitive electrochemical cocaine biosensor based on reversible DNA nanostructure. Biosens Bioelectron 51:191–194. https://doi.org/10.1016/j.bios.2013.07.053
The project was supported by the National Natural Science Foundation of China (grant no. 31701688, 21707070), and the Natural Science Foundation of Jiangsu Province of China (grant no. BK20170998).
The author(s) declare that they have no competing interests.
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Chen, X., Shi, X., Liu, Y. et al. Impedimetric determination of Staphylococcal enterotoxin B using electrochemical switching with DNA triangular pyramid frustum nanostructure. Microchim Acta 185, 460 (2018). https://doi.org/10.1007/s00604-018-2983-3
- Triangular pyramid frustum nanostructure
- Staphylococcus aureus
- Electrochemical biosensor
- Gold electrode
- Electron transfer
- Double-layer barrier
- Faradaic impedance