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A chemiluminescence-based catalase assay using H2O2-sensitive CdTe quantum dots

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Abstract

A method is described for the chemiluminescence based determination of the activity of catalase (CAT) using H2O2-sensitive CdTe quantum dots (QDs). It is based on the finding that the chemiluminescence (CL) of the CdTe/H2O2 system is reduced due to the consumption of H2O2 by the catalytic action of CAT. The Michaelis constant is calculated to be 519 ± 27 mM, showing the potential of the method to accurately measure the Km compared to the standard method. The method does not require QDs to be conjugated to biological/organic molecules and therefore is considered to be a rapid and convenient method for determination of CAT in real samples. At an incubation time of 2 s, the LOD was calculated to be 4.5 unit/mL, with a linear range from 6 to 400 unit/mL. The assay is sensitive, simple, and suitable for practical applications.

Schematic representation of chemiluminescence-based catalase U(CAT) assay using the CdSe QD/H2O2 system. The reduction of H2O2 is reflected by the chemiluminescence of the QDs. A mechanism is put forward based on the changes in chemiluminescence intensity of the QDs by the consumption of H2O2 due to the catalytic action of CAT.

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Acknowledgments

The authors express their gratitude to the research council of Tarbiat Modares University and Ministry of Sciences, Researches, and Technology for financial support during the course of this project. We also thank Hossein Rahmani for his guidance with this project.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, 14115-154, Iran

    Fahimeh Ghavamipour, Reza H. Sajedi & Khosro Khajeh

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  1. Fahimeh Ghavamipour
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  2. Reza H. Sajedi
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  3. Khosro Khajeh
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Correspondence to Reza H. Sajedi.

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Ghavamipour, F., Sajedi, R.H. & Khajeh, K. A chemiluminescence-based catalase assay using H2O2-sensitive CdTe quantum dots. Microchim Acta 185, 376 (2018). https://doi.org/10.1007/s00604-018-2912-5

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  • DOI: https://doi.org/10.1007/s00604-018-2912-5

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