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Aptamer-based fluorometric lateral flow assay for creatine kinase MB

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Abstract

A group of aptamers possessing high specificity and affinity for creatine kinase MB (CKMB) was obtained by magnetic systematic evolution of ligands by exponential enrichment. Two aptamers (referred to as C.Apt.21 and C.Apt.30) were found to possess adequately low Kd values. They form a well suited pair for CKMB binding. By using fluorescent microspheres, an aptamer-based lateral flow assay was developed. It is portable, economical, and sensitive. The limit of detection for CKMB is as low as 0.63 ng·mL-1, and the assay works in the 0.005 - 2 μg·mL-1 CKMB concentration range. The method is specific for CKMB, and biomarkers for AMI (such as cardiac troponin I and myoglobin) and serum do not interfere. The strip is highly accurate as shown by analysis of spiked serum samples which gave recoveries ranging between 88 and 117%.

Schematic of the test strip and sandwich aptamer-based fluorometric lateral flow assay for creatine kinease. The detection is based on the specific affinity between CKMB and selected aptamers to form a sandwich structure.

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Acknowledgement

This work was partly supported by the National Special R&D Programmer for Key Scientific Instruments and Equipment (2012YQ04014001) and the Beijing Institute of Technology Research Foundation (3160050321211).

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Authors and Affiliations

  1. Beijing Key Laboratory for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Life Science, Beijing Institute of Technology, 5 South Zhongguancun Street, Haidian District, Beijing, 100081, People’s Republic of China

    Jing Zhang, Xuefei Lv, Wei Feng, Xiaoqiong Li, Kunjie Li & Yulin Deng

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  1. Jing Zhang
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  2. Xuefei Lv
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Correspondence to Xiaoqiong Li.

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Zhang, J., Lv, X., Feng, W. et al. Aptamer-based fluorometric lateral flow assay for creatine kinase MB. Microchim Acta 185, 364 (2018). https://doi.org/10.1007/s00604-018-2905-4

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