Dye sensitized photoelectrochemical immunosensor for the tumor marker CEA by using a flower-like 3D architecture prepared from graphene oxide and MoS2
- 227 Downloads
The authors describe a dye-sensitized photoelectrochemical immunoassay for the tumor marker carcinoembryonic antigen (CEA). The method employs the rhodamine dye Rh123 with red color and absorption maximum at 500 nm for spectral sensitization, and a 3D nanocomposite prepared from graphene oxide and MoS2 acting as the photoelectric conversion layer. The nanocomposite with flower-like 3D architectures was characterized by transmission electron microscopy, scanning electron microscopy, X-ray powder diffraction, and UV-vis diffuse reflectometry. A photoelectrochemical sandwich immunoassay was developed that is based on the use of the nanocomposite and based on the specific binding of antibody and antigen, and by using a secondary antibody labeled with Rh123 and CdS (Ab2-Rh123@CdS). Under optimal conditions and at a typical working voltage of 0 V (vs. Hg/HgCl2), the photocurrent increases linearly 10 pg mL−1 to 80 ng mL−1 CEA concentration range, with a 3.2 pg mL−1 detection limit.
KeywordsImmunoassay Sandwich-type Signal amplification Sensitization Two-dimension nanomaterials
This work was supported by the National Natural Science Foundation of China (Grant No. 21605111 and 21706178), and Natural Science Foundation of Shanxi Province (No. 201601D021037 and 201601D011079).
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 13.Shen Y, Reparaz JS, Wagner MR, Hoffmann A, Thomsen C, Lee J, Heeg S, Hatting B, Reich S, Saeki A, Seki S, Yoshida K, Babu SS, Mohwald H, Nakanishi T (2011) Assembly of carbon nanotubes and alkylated fullerenes: nanocarbon hybrid towards photovoltaic applications. Chem Sci 2:2243–2250CrossRefGoogle Scholar
- 14.Lv Y, Chen S, Shen Y, Ji J, Zhou Q, Liu S, Zhang Y (2018) Competitive Multiple-Mechanism-Driven Electrochemiluminescent Detection of 8-Hydroxy-2′-deoxyguanosine. J Am Chem Soc 14:2801–2804Google Scholar