Microchimica Acta

, 185:302 | Cite as

Ionic liquid coated iron nanoparticles are promising peroxidase mimics for optical determination of H2O2

  • Faiza Zarif
  • Sajid Rauf
  • Muhammad Zahid Qureshi
  • Noor Samad Shah
  • Akhtar Hayat
  • Nawshad Muhammad
  • Abdur Rahim
  • Mian Hasnain Nawaz
  • Muhammad Nasir
Original Paper


Ionic liquid coated nanoparticles (IL-NPs) consisting of zero-valent iron are shown to display intrinsic peroxidase-like activity with enhanced potential to catalyze the oxidation of the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide. This results in the formation of a blue green colored product that can be detected with bare eyes and quantified by photometry at 652 nm. The IL-NPs were further doped with bismuth to enhance its catalytic properties. The Bi-doped IL-NPs were characterized by FTIR, X-ray diffraction and scanning electron microscopy. A colorimetric assay was worked out for hydrogen peroxide that is simple, sensitive and selective. Response is linear in the 30–300 μM H2O2 concentration range, and the detection limit is 0.15 μM.

Graphical abstract

Schematic of ionic liquid coated iron nanoparticles that display intrinsic peroxidase-like activity. They are capable of oxidizing the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide. This catalytic oxidation generated blue-green color can be measured by colorimetry. Response is linear in the range of 30–300 μM H2O2 concentration, and the detection limit is 0.15 μM.


Zero-valent iron particles Bismuth doping Ionic liquid coated nanoparticles TMB oxidation Stabilizing agent Peroxidase mimic Visual detection Hydrogen peroxide 



This work has been supported by IRCBM, COMSATS Institute of Information Technology Lahore, Pakistan.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_2841_MOESM1_ESM.doc (942 kb)
ESM 1 (DOC 942 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Faiza Zarif
    • 1
  • Sajid Rauf
    • 2
  • Muhammad Zahid Qureshi
    • 1
  • Noor Samad Shah
    • 3
  • Akhtar Hayat
    • 2
  • Nawshad Muhammad
    • 2
  • Abdur Rahim
    • 2
  • Mian Hasnain Nawaz
    • 2
  • Muhammad Nasir
    • 2
  1. 1.Department of ChemistryGovernment College UniversityLahorePakistan
  2. 2.Interdisciplinary Research Centre in Biomedical Materials (IRCBM) COMSATS Institute of Information TechnologyLahorePakistan
  3. 3.Department of Environmental SciencesCOMSATS Institute of Information TechnologyVehariPakistan

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