Microchimica Acta

, 185:291 | Cite as

Colorimetric determination of glutathione by using a nanohybrid composed of manganese dioxide and carbon dots

  • Qian Wang
  • Hongchang Pang
  • Yongqiang Dong
  • Yuwu Chi
  • Fengfu Fu
Original Paper


A kind of single-layer carbon based dots (CDs) with abundant carboxyl functional groups was hybridized with manganese dioxide (MnO2). The resulting nanohybrid is stable and can be well dispersed in water. MnO2 is capable of oxidizing the substrate 3,3′5,5′-tetramethylbenzidine (TMB) to form a blue product whose absorption (peaking at 655 nm) fades in the presence of glutathione (GSH). A sensitive and selective colorimetric GSH assay was worked out that has a linear response in the 10 to 0.1 µM GSH concentration range, with a 0.095 μM detection limit. The method was applied to the determination of GSH in spiked fetal calf serum where it gave excellent recoveries.

Graphical abstract

Schematic of the preparation of a nanohybrid composed of manganese dioxide and carbon based dots (MnO2/CDs). They can be used for the colorimetric detection of glutathione (GSH) based on the color change of 3,3′5,5′-tetramethylbenzidine (TMB).


Manganese dioxide/carbon based dots nanohybrids Oxidation Tetramethylbenzidine Fatel calf serum sample Visual detection 



This study was financially supported by National Key Research and Development Program of China (2017YFC1600500), National Natural Science Foundation of China (21675026, 21675027, 21506029), Natural Science Foundation of Fujian Province (2017Y01010023).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_2830_MOESM1_ESM.docx (48 kb)
ESM 1 (DOCX 47 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.MOE Key Laboratory of Analysis and Detection Technology for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, and Department of ChemistryFuzhou UniversityFujianChina
  2. 2.State Key Laboratory of Fine Chemicals, School of Chemical EngineeringDalian University of TechnologyDalianChina

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