Microchimica Acta

, 185:300 | Cite as

Polydopamine-based molecularly imprinting polymers on magnetic nanoparticles for recognition and enrichment of ochratoxins prior to their determination by HPLC

  • Meihua Hu
  • Pengcheng Huang
  • Lili Suo
  • Fangying Wu
Original Paper


A polydopamine-based molecularly imprinted polymer was deposited on the surface of magnetite (ferroferric oxide) nanoparticles (Fe3O4@PDA MIPs) and is shown to be an efficient and fairly specific sorbent for the extraction of various ochratoxins. The MIPs were characterized by IR spectroscopy and transmission electron microscopy. The adsorption capacities, evaluated through the langmuir adsorption isotherm model, are 1.8, 0.23 and 0.17 mg·g−1 for ochratoxin A, ochratoxin B and ochratoxin C, respectively. Parameters such as the amount of magnetic MIPs, pH value, time for ultrasonication, elution solvent and volume were optimized. Following desorption from the MIP with acetonitrile, the ochratoxins were quantified by HPLC with fluorometric detection. Under optimal experimental conditions, the calibration plots are linear in the range of 0.01–1.0 ng·mL−1 of OTA, 0.02–2.0 ng·mL−1 of OTB, and 0.002–0.2 ng·mL−1 of OTC. The LODs are between 1.8 and 18 pg·mL−1, and the recoveries from spiked samples are 71.0% - 88.5%, with RSDs of 2.3–3.8% in case of rice and wine samples. The MIPs can be re-used for at least 7 times.

Graphical abstract

Schematic of the preparation of a magnetic molecularly imprinted polymer based on self-polymerization of dopamine in weakly alkaline solution. Ochratoxins are recognized owing to homologous cavities in the MIPs, and quantified by HPLC after desorption with acetonitrile.


Rice Wine Separation Fe3O4 Template Langmuir adsorption isotherm Toxin 



We greatly acknowledge the National Natural Science Foundation of China (No. 21765014 and 21505067) for financial support.

Compliance with ethical standards

The authors declare that they have no competing interests.

Supplementary material

604_2018_2826_MOESM1_ESM.doc (764 kb)
ESM 1 (DOC 763 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Meihua Hu
    • 1
    • 2
  • Pengcheng Huang
    • 1
  • Lili Suo
    • 2
  • Fangying Wu
    • 1
  1. 1.College of ChemistryNanchang UniversityNanchangPeople’s Republic of China
  2. 2.Physical and Chemical DepartmentNanchang Centre for Disease Control and PreventionNanchangPeople’s Republic of China

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