Amorphous titania modified with boric acid for selective capture of glycoproteins
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Amorphous titania was modified with boric acid, and the resulting material was characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray powder diffraction and X-ray photoelectron spectrometry. The new material, in contrast to conventional boronate affinity materials containing boronic acid ligands, bears boric acid groups. It is shown to exhibit high specificity for glycoproteins, and this was applied to design a method for solid phase extraction of glycoproteins as shown for ribonuclease B, horse radish peroxidase and ovalbumin. Glycoproteins were captured under slightly alkaline environment and released in acidic solutions. The glycoproteins extracted were detected by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The binding capacities for ribonuclease B, horse radish peroxidase and ovalbumin typically are 9.3, 26.0 and 53.0 mg ∙ g−1, respectively. The method was successfully applied to the selective enrichment of ovalbumin from egg white.
KeywordsBoronate affinity Solid phase extration Ovalbumin Horse radish peroxidase Ribonuclease B
This work has been supported by the Natural Science Foundation of Hubei Province of China (no. 2014CFB179).
Compliance with ethical standards
The authors declare that they have no competing interests.
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