Gold nanocluster-based ratiometric fluorescent probes for hydrogen peroxide and enzymatic sensing of uric acid
A method is described for ratiometric fluorometric assays of H2O2 by using two probes that have distinct response profiles. Under the catalytic action of ferrous ion, the 615 nm emission of protein-stabilized gold nanoclusters (under 365 nm photoexcitation) is quenched by H2O2, while an increased signal is generated with a peak at 450 nm by oxidizing coumarin with the H2O2/Fe(II) system to form a blue emitting fluorophore. These decrease/increase responses give a ratiometric signal. The ratio of the fluorescences at the two peaks are linearly related to the concentration of H2O2 in the range from 0.05 to 10 μM, with a 7.7 nM limit of detection. The detection scheme was further coupled to the urate oxidase catalyzed oxidation of uric acid which proceeds under the formation of H2O2. This method provides an simple and effective means for the construction of ratiometric fluorometric (enzymatic) assays that involve the detection of H2O2.
KeywordsCoumarin Hydroxy radical Oxidation Urate oxidase Catalysis Fenton reaction
This work was supported by the Natural Science Foundation of China (No. 21305100), the Project of Scientific and Technologic Infrastructure of Suzhou (SZS201208) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 1.Gu KZ, Xu YS, Li H, Guo ZQ, Zhu SJ, Zhu SQ, Shi P, James TD, Tian H, Zhu WH (2016) Real-time tracking and in vivo visualization of β-galactosidase activity in colorectal tumor with a ratiometric near-infrared fluorescent probe. J Am Chem Soc 138:5334–5340. https://doi.org/10.1021/jacs.6b01705 CrossRefGoogle Scholar
- 3.Shojaeifard Z, Hemmateenejad B, Shamsipur M (2016) Efficient on−off ratiometric fluorescence probe for cyanide ion based on perturbation of the interaction between gold nanoclusters and a copper(ii)-phthalocyanine complex. ACS Appl Mater Inter 8:15177–15186. https://doi.org/10.1021/acsami.6b01566 CrossRefGoogle Scholar
- 6.Ma YS, Cen Y, Sohail M, Xu GH, Wei FD, Shi ML, Xu XM, Song YY, Ma YJ, Hu Q (2017) A ratiometric fluorescence universal platform based on N, Cu codoped carbon dots to detect metabolites participating in H2O2-generation reactions. ACS Appl Mater Inter 9:33011–33019. https://doi.org/10.1021/acsami.7b10548 CrossRefGoogle Scholar
- 7.Zhang J, Zhu XY, Hu XX, Liu HW, Li J, Feng LL, Yin X, Zhang XB, Tan WH (2016) Ratiometric two-photon fluorescent probe for in vivo hydrogen polysulfides detection and imaging during lipopolysaccharide-induced acute organs injury. Anal Chem 88:11892–11899. https://doi.org/10.1021/acs.analchem.6b03702 CrossRefGoogle Scholar
- 18.Kumar JV, Karthik R, Chen SM, Raja N, Selvam V, Muthuraj V (2017) Evaluation of a new electrochemical sensor for selective detection of non-enzymatic hydrogen peroxide based on hierarchical nanostructures of zirconium molybdate. J Colloid Interf Sci 500:44–53. https://doi.org/10.1016/j.jcis.2017.03.113 CrossRefGoogle Scholar
- 24.Deng HH, Wu GW, He D, Peng HP, Liu AL, Xia XH, Chen W (2015) Fenton reaction-mediated fluorescence quenching of N-acetyl-L-cysteine-protected gold nanoclusters: analytical applications of hydrogen peroxide, glucose, and catalase detection. Analyst 140:7650–7656. https://doi.org/10.1039/C5AN01284H33 CrossRefGoogle Scholar
- 25.Pradhan S, Das R, Biswas S, Das DK, Bhar R, Bandyopadhyay R, Pramanik P (2017) Chemical synthesis of nanoparticles of nickel telluride and cobalt telluride and its electrochemical applications for determination of uric acid and adenine. Electrochim Acta 238:185–193. https://doi.org/10.1016/j.electacta.2017.04.023 CrossRefGoogle Scholar
- 26.Wang J, Yang BB, Zhong JT, Yan B, Zhang K, Zhai CY, Shiraishi Y, Du YK, Yang P (2017) Dopamine and uric acid electrochemical sensor based on a glassy carbon electrode modified with cubic Pd and reduced graphene oxide nanocomposite. J Colloid Interf Sci 497:172–180. https://doi.org/10.1016/j.jcis.2017.03.011 CrossRefGoogle Scholar
- 31.Yadav DK, Gupta R, Ganesan V, Sonkar PK (2017) Individual and simultaneous voltammetric determination of ascorbic acid, uric acid and folic acid by using a glassy carbon electrode modified with gold nanoparticles linked to bentonite via cysteine groups. Microchim Acta 184:1951–1957. https://doi.org/10.1007/s00604-017-2186-3 CrossRefGoogle Scholar
- 32.Yan SL, Li X, Xiong Y, Wang MM, Yang LL, Liu X, Li XY, Alshahrani LAM, Liu P, Zhang CC (2016) Simultaneous determination of ascorbic acid, dopamine and uric acid using a glassy carbon electrode modified with the nickel(II)-bis(1,10-phenanthroline) complex and single-walled carbon nanotubes. Microchim Acta 183:1401–1408. https://doi.org/10.1007/s00604-016-1776-9 CrossRefGoogle Scholar
- 33.Dai HX, Wang N, Wang DL, Zhang XM, Ma HY, Lin M (2016) Voltammetric uric acid sensor based on a glassy carbon electrode modified with a nanocomposite consisting of polytetraphenylporphyrin, polypyrrole, and graphene oxide. Microchim Acta 183:3053–3059. https://doi.org/10.1007/s00604-016-1953-x CrossRefGoogle Scholar