Microchimica Acta

, 185:305 | Cite as

Gold nanocluster-based ratiometric fluorescent probes for hydrogen peroxide and enzymatic sensing of uric acid

  • Dongqin Yang
  • Minchuan Luo
  • Junwei Di
  • Yifeng Tu
  • Jilin Yan
Original Paper


A method is described for ratiometric fluorometric assays of H2O2 by using two probes that have distinct response profiles. Under the catalytic action of ferrous ion, the 615 nm emission of protein-stabilized gold nanoclusters (under 365 nm photoexcitation) is quenched by H2O2, while an increased signal is generated with a peak at 450 nm by oxidizing coumarin with the H2O2/Fe(II) system to form a blue emitting fluorophore. These decrease/increase responses give a ratiometric signal. The ratio of the fluorescences at the two peaks are linearly related to the concentration of H2O2 in the range from 0.05 to 10 μM, with a 7.7 nM limit of detection. The detection scheme was further coupled to the urate oxidase catalyzed oxidation of uric acid which proceeds under the formation of H2O2. This method provides an simple and effective means for the construction of ratiometric fluorometric (enzymatic) assays that involve the detection of H2O2.

Graphical abstract

Under catalysis by ferrous ion, hydrogen peroxide quenches the luminescence of gold nanoclusters (AuNCs) and oxidizes coumarin into a fluorescent derivative, which rendered fluorescence ON and OFF at two distinct wavelengths for ratiometric measurements.


Coumarin Hydroxy radical Oxidation Urate oxidase Catalysis Fenton reaction 



This work was supported by the Natural Science Foundation of China (No. 21305100), the Project of Scientific and Technologic Infrastructure of Suzhou (SZS201208) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_2823_MOESM1_ESM.docx (271 kb)
ESM 1 (DOCX 271 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Dongqin Yang
    • 1
  • Minchuan Luo
    • 1
  • Junwei Di
    • 1
  • Yifeng Tu
    • 1
  • Jilin Yan
    • 1
  1. 1.The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials ScienceSoochow UniversitySuzhouChina

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