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Monodisperse magnetic poly(glycidyl methacrylate) microspheres for isolation of autoantibodies with affinity for the 46 kDa form of unconventional Myo1C present in autoimmune patients


Monodisperse nonmagnetic macroporous poly(glycidyl methacrylate) (PGMA) microspheres were synthesized by multistep swelling polymerization of glycidyl methacrylate, ethylene dimethacrylate and 2-[(methoxycarbonyl)methoxy]ethyl methacrylate (MCMEMA). This was followed (a) by ammonolysis to modify the microspheres with amino groups, and (b) by incorporation of iron oxide (γ-Fe2O3) into the pores to render the particles magnetic. The resulting porous and magnetic microspheres were characterized by scanning and transmission electron microscopy (SEM and TEM), atomic absorption and Fourier transform infrared spectroscopy (AAS and FTIR), elemental analysis, vibrating magnetometry, mercury porosimetry and Brunauer-Emmett-Teller adsorption/desorption isotherms. The microspheres are meso- and macroporous, typically 5 μm in diameter, contain 0.9 mM · g−1 of amino groups and 14 wt.% of iron according to elemental analysis and AAS, respectively. The particles were conjugated to p46/Myo1C protein, a potential biomarker of autoimmune diseases, to isolate specific autoantibodies in the blood of patients suffering from multiple sclerosis (MS). The p46/Myo1C loaded microspheres are shown to enable the preconcentration of minute quantities of specific immunoglobulins prior to their quantification via SDS-PAGE. The immunoglobulin M (IgM) with affinity to Myo1C was detected in MS patients.

Monodisperse magnetic poly(glycidyl methacrylate) microspheres were synthesized, conjugated with 46 kDa form of unconventional Myo1C protein (p46/Myo1C) via carbodiimide (DIC) chemistry, and specific autoantibodies isolated from blood of multiple sclerosis (MS) patients; immunoglobulin M (IgM) level increased in MS patients.

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Atomic absorption spectroscopy


Brunauer-Emmett-Teller adsorption/desorption isotherm

d :

Pore diameter


Fourier transform infrared spectroscopy


Glycidyl methacrylate


2-[(Methoxycarbonyl)methoxy]ethyl methacrylate

mag-PGMA-NH2 :

Magnetic PGMA-NH2


Multiple sclerosis


Poly(glycidyl methacrylate)


Poly(glycidyl methacrylate) microspheres modified with amino groups


Specific surface area


Scanning electron microscopy


Sodium dodecyl sulfate


SDS polyacrylamide gel electrophoresis


Transmission electron microscopy.


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Support of LQ1604 NPU II provided by MEYS and CZ.1.05/1.1.00/02.0109 BIOCEV provided by ERDF and MEYS, the Cedars-Sinai Medical Center’s International Research and Innovation Management Program and the RECOOP HST Association, as well as Volkswagen Foundation Trilateral Partnership Ukraine, Russian Federation and Germany, and the Target Complex Interdisciplinary Research Program of the NAS of Ukraine (No. 32-16) is acknowledged. The authors thank to Ms. Jiřina Hromádková for the micrographs of the particles.

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Correspondence to Daniel Horák.

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Zasońska, B.A., Hlídková, H., Petrovský, E. et al. Monodisperse magnetic poly(glycidyl methacrylate) microspheres for isolation of autoantibodies with affinity for the 46 kDa form of unconventional Myo1C present in autoimmune patients. Microchim Acta 185, 262 (2018).

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  • Magnetic microspheres
  • Functionalization
  • Affinity chromatography
  • p46/Myo1C protein
  • Autoantibody
  • Immunoglobulin M
  • Autoimmune disease marker
  • Multiple sclerosis