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Microchimica Acta

, 185:250 | Cite as

Nonenzymatic determination of glucose at near neutral pH values based on the use of nafion and platinum black coated microneedle electrode array

  • Somasekhar R. Chinnadayyala
  • Ilhwan Park
  • Sungbo Cho
Original Paper

Abstract

The authors report on a microneedle-based amperometric nonenzymatic glucose sensor for painless and continuous monitoring of glucose. It consists of 3 × 5 sharp stainless steel microneedles micromachined from a stainless steel substrate. The microneedles are 600 and 100 μm in height and width, respectively. Nafion and platinum black were sequentially coated onto the tip of gold-coated microneedles and used for nonenzymatic (direct) sensing of glucose. Attractive features of the modified microneedle electrode include (a) a low working potential (+0.12 V vs. Ag/AgCl), (b) a linear response in the physiologically relevant range (1–40 mM), (c) a sensitivity as high as 175 μA mM−1 cm−2, (d) a 23 μM detection limit, and (e) a response time of 2 s. The sensor also exhibits good reproducibility and stability. The sensor is selective for glucose even in the presence of 10-fold higher concentrations of ascorbic acid, lactic acid, dopamine, uric acid, and acetaminophen.

Graphical abstract

Schematic representation of the fabrication sequence for a nonenzymatic electrochemical glucose sensor using Nafion and platinum black coated microneedle electrode array. The sensor is based on measuring the faradaic current at +0.12 V vs. Ag/AgCl by the direct electrochemical oxidation of glucose to gluconic acid on the surface of a Pt black sensing layer.

Keywords

Chloroplatinic acid hexahydrate Lead acetate Parylene Polydimethylsiloxane Electrochemical sensor Scanning electron microscope Chronoamperometry Cyclic voltammetry Electrodeposition 

Notes

Acknowledgments

The work was supported by the National Research Foundation of Korea (No. NRF-2017R1D1A1A09000712, 2018R1C1B6009385, 2018M3A9F1023690).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_2770_MOESM1_ESM.docx (728 kb)
ESM 1 (DOCX 727 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Somasekhar R. Chinnadayyala
    • 1
  • Ilhwan Park
    • 2
  • Sungbo Cho
    • 2
    • 3
  1. 1.Department of Biomedical EngineeringGachon UniversityIncheonRepublic of Korea
  2. 2.Gachon Advanced Institute for Health Science & TechnologyGachon UniversityIncheonRepublic of Korea
  3. 3.Department of Electronic EngineeringGachon UniversitySeongnam-siRepublic of Korea

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