A two-wavelength fluorescence recovery method for the simultaneous determination of aureomycin and oxytetracycline by using gold nanocrystals modified with serine and 11-mercaptoundecanoic acid
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A method is described for rapid (1 h) synthesis of gold nanoclusters (AuNCs) co-functionalized with serine and 11-mercaptoundecanoic acid. The co-functionalized AuNCs exhibit good stability towards temperature, pH values, and over time. They were characterized by atomic force microscopy, high-resolution transmission electron microscopy, dynamic light scattering, and by fluorescence, IR and X-ray photoelectron spectroscopies. The fluorescence of the AuNCs is quenched by Hg(II) and restored on subsequent addition of aureomycin (CTC) or oxytetracycline (OTC). A fluorescent turn-on assay was worked out for simultaneous detection of CTC and OTC based on recording the change of the restored fluorescence measured at 420 and 500 nm under 340 nm photoexcitation. The detection limits are 20 and 9 nM for CTC and OTC, respectively. The concentrations of CTC and OTC can also be visualized by UV illumination. The nanoprobe was successfully applied to the simultaneous determination of CTC and OTC in spiked human urine.
KeywordsGold nanoclusters Visual detection Fluorescence Antibiotics Quenching
S. Xu, Y. Mao, Y. Nie and J. Wang are grateful for the financialsupport provided by NSFC (21505081) and the Doctoral Found of QUST (010022832). X. Luo acknowledges the financial support by NSFC (21422504 and 21675093), the Natural Science Foundation of Shandong Province of China (JQ201406) and the Taishan Scholar Program of Shandong Province, China(ts20110829).
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 6.Wang Y, Gan N, Zhou Y, Li TH, Hu FT, Cao YT, Chen YJ (2017) Novel label-free and high-throughput microchip electrophoresis platform for multiplex antibiotic residues detection based on aptamer probes and target catalyzed hairpin assembly for signal amplification. Biosens Bioelectron 97:100–106CrossRefGoogle Scholar
- 14.Xu SH, Feng XY, Gao T, Liu GF, Mao YN, Lin JH, Yu XJ, Luo XL (2017) Aptamer induced multicoloured AuNCs-MoS2 “switch on” fluorescence resonance energy transfer biosensor for dual color simultaneous detection of multiple tumor markers by single wavelength excitation. Anal Chim Acta 983:173–180CrossRefGoogle Scholar
- 16.Bian PP, Zhou J, Liu YY, Ma ZF (2013) One-step fabrication of intense red fluorescent gold nanoclusters and their application in cancer cell imaging. Nano 5:6161–6166Google Scholar
- 21.Shang L, Dörlich RM, Brandholt S, Schneider R, Trouillet V, Bruns M, Gerthsen D, Nienhaus GU (2011) Facile preparation of water-soluble fluorescent gold nanoclusters for cellular imaging applications. Nano 3:2009–2014Google Scholar
- 28.Huang CC, Chang HT (2007) Parameters for selective colorimetric sensing of mercury (II) in aqueous solutions using mercaptopropionic acid-modified gold nanoparticles. Chem Commun (12):1215–1217Google Scholar
- 30.Chen W, Tu X, Guo X (2009) Fluorescent gold nanoparticles-based fluorescence sensor for Cu2+ ions. Chem Commun (13):1736–1738Google Scholar
- 31.Zheng J, Zhang C, Dickson RM (2004) Highly fluorescent, watersoluble, size-tunable gold quantum dots. Phys Rev Lett 93:0774021–0774024Google Scholar
- 35.Gaudin V (2017) Advances in biosensor development for the screening of antibiotic residues in food products of animal origin-A comprehensive review. 90: 363–377Google Scholar