Microchimica Acta

, 185:219 | Cite as

Voltammetric determination of 5-hydroxytryptamine based on the use of platinum nanoparticles coated with molecularly imprinted silica

  • Yiwen Yang
  • Yanbo Zeng
  • Chuangui Tang
  • Xudong Zhu
  • Xing Lu
  • Lingyu Liu
  • Zhidong Chen
  • Lei Li
Original Paper


The authors describe an electrochemical sensor for the determination of 5-hydroxytryptamine (5-HT; serotonin). It is based on the use of platinum nanoparticles (PtNPs) coated with molecularly imprinted silica (MIS). The MIS-coated PtNPs were prepared from 5-HT, PtNPs, phenyltrimethoxysilane and tetraethoxysilane that act as template, support, functional monomer and cross-linker, respectively. The MIS-coated PtNPs were characterized by Fourier transfer infrared spectroscopy and transmission electron microscopy. The MIS-coated PtNPs were drop-cast onto a glassy carbon electrode (GCE) to obtain an electrochemical sensor for 5-HT which exhibited fast response and high recognition ability for 5-HT. The imprinting factor of this electrode for 5-HT is 4.12, which is higher than that for its analogs. Under optimized conditions and at a typical working potential of 0.29 V (vs. Hg/Hg2Cl2), the electrode has a linear response in the 0.05–80 μM 5-HT concentration range and a 0.02 μM detection limit. The electrode was successfully applied to the determination of 5-HT in human serum samples.

Graphical Abstract

Molecularly imprinted silica-coated platinum nanoparticles were prepared using surface imprinting technique. A glassy carbon electrode modified with the surface imprinted material exhibits fast response in detecting 5-hydroxytryptamine, a low detection limit, good selectivity, reproducibility and stability.


Platinum nanoparticles Silica; surface molecular imprinting Molecularly imprinted polymer Sol-gel 5-Hydroxytryptamine Serotonin Electrochemical assay 



This work was supported by the National Natural Science Foundation of China (No. 21507041, 21677060), the Zhejiang Provincial Natural Science Foundation of China under Grant No. LY16B050007 and LQ14B050002, the Program for Public technology of Zhejiang Province (No. LGF18B050004), and the Program for Science and Technology of Jiaxing (2017AY33034).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_2755_MOESM1_ESM.doc (5.9 mb)
ESM 1 (DOC 6024 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Biological, Chemical Sciences and EngineeringJiaxing UniversityJiaxingPeople’s Republic of China
  2. 2.School of Petrochemical EngineeringChangzhou UniversityChangzhouPeople’s Republic of China
  3. 3.College of Chemistry and Life ScienceZhejiang Normal UniversityJinhuaPeople’s Republic of China

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