A voltammetric immunosensor for clenbuterol based on the use of a MoS2-AuPt nanocomposite
- 178 Downloads
An ultrasensitive immunosensor for the direct detection of the illegally used livestock feed clebuterol (CLB) is described. It is based on the use of a glassy carbon electrode modified with an MoS2-AuPt nanocomposite and on biotin-streptavidin interaction. The use of MoS2-AuPt accelerates electron transfer, and this leads to a sharp increase in the electrochemical signal for the electrochemical probe hydrogen peroxide. Differential pulse voltammetry was used to record the current signal at a peak potential of −0.18 V (vs SCE). Under optimal conditions, the electrode has a linear response in the 10 pg·mL−1 to 100 ng·mL−1 CLB concentration range and a 6.9 pg·mL−1 detection limit (based on the 3σ criterium). This immunosensor is sensitive, highly specific and acceptably reproducible, and thus represents a valuable tool for the determination of CLB in pork.
Keywordsβ-Adrenergic agents Biotin-streptavidin system Differential pulse voltammetry Food safety Signal amplification
This research was supported by the National Natural Science Foundation of China (Grant Nos. 31071093, 31170129, and 31200064) and the Science and Technology Planning Project of Yuzhong District of Chongqing City, China (No. 20140119).
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 4.Yan F, Zhang Y, Zhang S, Zhao J, Liu S, He L, Feng X, Zhang H, Zhang Z (2015) Carboxyl-modified graphene for use in an immunoassay for the illegal feed additive clenbuterol using surface plasmon resonance and electrochemical impedance spectroscopy. Microchim Acta 182(3–4):855–862CrossRefGoogle Scholar
- 5.Yang Y, Zhang H, Huang C, Yang D, Jia N (2016) Electrochemical non-enzyme sensor for detecting clenbuterol (CLB) based on MoS 2 -Au-PEI-hemin layered nanocomposite. Biosens Bioelectron 89(Pt 1):461Google Scholar
- 6.Chen XB, Wu YL, Yang T (2011) Simultaneous determination of clenbuterol, chloramphenicol and diethylstilbestrol in bovine milk by isotope dilution ultraperformance liquid chromatography-tandem mass spectrometry. J Instrum Anal 879(11):799–803Google Scholar
- 34.Yang T, Chen M, Kong Q, Luo X, Jiao K (2016) Toward DNA electrochemical sensing by free-standing ZnO nanosheets grown on 2D thin-layered MoS2. Biosens Bioelectron 89(Pt 1):538–544Google Scholar