Microchimica Acta

, 185:210 | Cite as

Gold nanoparticle-based colorimetric ELISA for quantification of ractopamine

  • Shuaijuan Han
  • Tianjiao Zhou
  • Bingjie Yin
  • Pingli He
Original Paper
  • 116 Downloads

Abstract

The work describes a gold nanoparticle-based colorimetric enzyme-linked immunosorbent assay (ELISA) for ractopamine. The ELISA is based on an indirect competitive approach. In the presence of ractopamine, gold(III) ions are oxidized by H2O2 to form red AuNPs. On the other hand, the AuNP in solution are purple-blue due to aggregation if the sample does not contain ractopamine. The absorption, best measured at 560 nm, increases linearly in the 2 to 512 ng·mL−1 ractopamine concentration range, and the detection limit is as low as 0.35 ng·mL−1 in urine. Ractopamine can also be detected visually, even in the presence of other β-agonists and antibiotics. The results obtained by this method are consistent with those obtained by LC-MS/MS as demonstrated by analysis of sheep urine. The ELISA method described here is inexpensive, easy-to-use, and suitable for rapid screening of ractopamine in animal samples.

Graphical abstract

Schematic presentation of a colorimetric indirect competitive immunoassay for ractopamine. It is based on the use of catalase labeled IgG and the measurement of the absorption of red gold nanoparticles (AuNPs) that are generated by the reaction of gold ions with H2O2. In the absence of ractopamine, the solution becomes blue.

Keywords

β-Agonist Colorimetric assay Nanogold Visual test Catalase Plasma-assisted method Sheep urine Immunoassay Enzyme-linked immunosorbent assay 

Notes

Acknowledgements

The Natural Science Foundation of China (31472126) is gratefully acknowledged.

Compliance with ethical standards

The author(s) declare that they have no competing interests

Supplementary material

604_2018_2736_MOESM1_ESM.doc (452 kb)
ESM 1 (DOC 451 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.State Key Laboratory of Animal Nutrition, College of Animal Science and TechnologyChina Agricultural UniversityBeijingPeople’s Republic of China

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