Amperometric determination of myo-inositol by using a glassy carbon electrode modified with molecularly imprinted polypyrrole, reduced graphene oxide and nickel nanoparticles
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This paper reports on the development of an amperometric method for the determination of myo-inositol. The method involves coating of a glassy carbon electrode (GCE) with a molecularly imprinted polymer (MIP) and reduced graphene oxide (RGO) that was modified with nickel nanoparticles (NiNPs). The MIP was prepared by electropolymerization of pyrrole on the surface of the GCE in the presence of myo-inositol molecules. The construction steps of the modified electrode were monitored via cyclic voltammetry, atomic force microscopy, scanning electron microscopy and X-ray Photoelectron Spectroscopy. The results were evaluated using differential pulse voltammetry, in which hexacyanoferrate was used as an electrochemically active probe. Under optimized experimental conditions, the imprint-modified GCE has a linear response in the 1.0 × 10−10 mol L−1 to 1.0 × 10−8 mol L−1 concentration range, with a 7.6 × 10−11 mol L−1 detection limit and an electrochemical sensitivity of 4.5 μA·cm-2 μmol−1. The method showed improved selectivity even in the presence of molecules with similar chemical structure. The GCE modified was successfully applied to the determination of myo-inositol in sugarcane vinasse where it yielded recoveries that ranged from 95 to 102%.
KeywordsMolecularly imprinted polymer Metallic nanoparticle Pyrrole Electropolymerization Electrochemical determination Polyol compounds
Our sincerest gratitude to the Brazilian Research Funding Agencies – São Paulo Research Foundation (FAPESP) process n° 2014/23846-5 and Coordination for the Improvement of Higher Education Personnel (process n° 33004030072p8) for the financial support granted in the course of this research.
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 1.Holub BJ (1982) The nutritional significance, metabolism, and function of myo-inositol and phosphatidylinositol in health and disease. In: Advances in Nutritional Research Springer, Boston, pp 107–141Google Scholar
- 4.Pande M, Seal A, Mishra S, Dasgupta A, Sengupta M, Dastider R (2017) The effects of combined therapy of myo-inositol and D-chiro inositol in reduction of the individual components of metabolic syndrome in overweight PCOS patients compared to myo-inositol supplementation alone: a prospective randomised controlled trial. Int J Reprod Contraception, Obstet Gynecol 6:2939–2943CrossRefGoogle Scholar
- 6.Marjańska M, Weigand SD, Preboske G, Wengenack TM, Chamberlain R, Curran GL, Poduslo JF, Garwood M, Kobayashi D, Lin JC, Jack CR (2014) Treatment effects in a transgenic mouse model of Alzheimer’s disease: a magnetic resonance spectroscopy study after passive immunization. Neuroscience 259:94–100CrossRefGoogle Scholar
- 32.Portal Brasil (2017) Safra 2017/2018 de cana-de-açúcar deve ser de 647 milhões de toneladas. http://www.brasil.gov.br/economia-e-emprego/2017/04/safra-2017-2018-de-cana-de-acucar-deve-ser-de-647-milhoes-de-toneladas. Accessed 1 Aug 2017