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Impedimetric detection of cocaine by using an aptamer attached to a screen printed electrode modified with a dendrimer/silver nanoparticle nanocomposite

Microchimica Acta volume 185, Article number: 214 (2018) Cite this article

Abstract

The authors describe a highly sensitive method for the aptamer (Apt) based impedimetric determination of cocaine. The surface of a screen-printed electrode (SPE) was modified with a nanocomposite of dendrimer and silver nanoparticles (AgNPs). The cocaine-binding Apt was attached to a dendrimer/AgNP/SPE surface, forming a sensitive layer for the determination of cocaine. The incubation with the analyte resulted in the formation of a cocaine/Apt complex on the electrode surface. As a consequence, folding and conformational change in the aptamer structure was induced, this resulting in a change in the impedimetric signal. The aptaassay exhibits highly efficient sensing characteristics with a good linearity of 1 fmol L−1 to 100 nmol L−1 (with two linear ranges) and a limit of detection (LOD) of 333 amol L−1. Its excellent specificity and high sensitivity suggest that this kind of aptamer-based assay may be applied to detect other targets in this field.

Designing of an aptaassay via immobilization of a functionalized aptamer with silver nanoparticle (AgNPs-Apt) on the modified screen-printed electrode (SPE) with dendrimer/silver nanoparticle nanocomposite (Den-AgNPs) for impedimetric detection of cocaine.

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Acknowledgements

The authors thank the Iran National Science Foundation (Grant no. 94808064) for their support. They would also like to thank the Ilam University Research Council and the Iranian Nanotechnology Initiative Council for their partial support.

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  1. Department of Chemistry, University of Ilam, Ilam, 69315-516, Iran

    Mahmoud Roushani & Faezeh Shahdost-fard

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  1. Mahmoud Roushani
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  2. Faezeh Shahdost-fard
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Correspondence to Mahmoud Roushani.

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The authors declare that they have no competing interests.

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Roushani, M., Shahdost-fard, F. Impedimetric detection of cocaine by using an aptamer attached to a screen printed electrode modified with a dendrimer/silver nanoparticle nanocomposite. Microchim Acta 185, 214 (2018). https://doi.org/10.1007/s00604-018-2709-6

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  • DOI: https://doi.org/10.1007/s00604-018-2709-6

Keywords

  • Electrochemical impedance spectroscopy
  • Nanomaterial
  • Aptasensor
  • Metal nanoparticles
  • Charge transfer resistance
  • Poly amidoamine
  • Chemisorption