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Selective extraction of theophylline from plasma by copper-doped magnetic microspheres prior to its quantification by HPLC

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Abstract

The authors describe the preparation of copper-doped magnetic microspheres (Cu-Fe3O4) by a solvothermal method. Due to their good magnetic property and high affinity for compounds containing an imidazole moiety (containing N-H), they are excellent adsorbents for such compounds as tested by eighteen compounds. Specifically, a method has been developed for magnetic solid-phase extraction (MSPE) of theophylline (TP) from plasma. The method enables selective enrichment of TP over many potential interferents that can occur in plasma. Following elution with alkaline methanol, TP was quantified by HPLC-UV at a detection wavelength of 272 nm. Under the optimized conditions, a linear response is found for the 0.02 to 20 μg·mL−1 concentration range, and the limit of detection is as low as 3 ng·mL−1. Recoveries from spiked samples range from 91.2 to 100.4%, and the repeatabilities are between 2.9 and 12% (for n = 6). The method was successfully applied to the determination of TP in rabbit and rat plasma.

Copper-doped magnetic microspheres are described that show good magnetic property and high affinity for compounds containing an imidazole moiety (containing an N-H group). They were successfully applied to the selective extraction of theophylline in plasma.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 21475104, 21775121 and 21575114) and the Industry Development Project by the Science and Technology Department of Shaanxi Province (No. 2016GY-214).

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Authors and Affiliations

  1. Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Key Laboratory of Modern Separation Science in Shaanxi Province, College of Chemistry & Materials Science, Northwest University, Xi’an, 710127, China

    Peihong Chen, Jiwei Shen, Chaozhan Wang & Yinmao Wei

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  1. Peihong Chen
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  2. Jiwei Shen
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  3. Chaozhan Wang
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  4. Yinmao Wei
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Correspondence to Yinmao Wei.

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Chen, P., Shen, J., Wang, C. et al. Selective extraction of theophylline from plasma by copper-doped magnetic microspheres prior to its quantification by HPLC. Microchim Acta 185, 113 (2018). https://doi.org/10.1007/s00604-017-2667-4

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