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Microchimica Acta

, 185:3 | Cite as

Aptamer based vanillin sensor using an ion-sensitive field-effect transistor

  • Alexander KuznetsovEmail author
  • Natalia Komarova
  • Maria Andrianova
  • Vitaliy Grudtsov
  • Evgeniy Kuznetsov
Original Paper

Abstract

An aptamer for vanillin was obtained and then used for the development of an aptasensor based on an ion-sensitive field-effect transistor (ISFET). This aptamer (a single-stranded DNA;ssDNA) was selected using the Capture-SELEX protocol, which suites well for selection of aptamers to small molecules. Among six aptamer candidates, the aptamer Van_74 with the highest affinity for vanillin was chosen (elution of 35% of the aptamer from a solid support in the presence of 2 mM of vanillin). Van_74 was characterized using nondenaturating PAGE of washouts from magnetic beads. It is shown that Van_74 binds to vanillin with an dissociation constant of >7.8 μM (determined by nondenaturating PAGE) and it was specific to vanillin in comparison with interferents: benzaldehyde, guaiacol, furaneol, ethyl guaiacol and ethyl vanillin. Also it was shown that change of buffer composition greatly affected the binding ability of Van_74. For biosensor fabrication aptamer was immobilised on the Ta2O5-sensitive surface of the ISFET via “click-chemistry”. Detection scheme implied dehybridisation of the ssDNA probe from the aptamer and release in the solution during the addition of vanillin. As a result, the surface potential increase upon vanillin binding with the aptamer was detected by the transistor. The biosensor had a detection limit of 1.55 × 10−7 M and a dynamic range from 1.55 × 10−7 M to 1 × 10−6 M. Effective constant Kd,eff for vanillin binding on biosensor surface was calculated to be (9 ± 3) × 10−7 M. This allows selective detection of vanillin in the mixture of interferents and in samples of coffee extract.

Graphical abstract

A biosensor for vanillin was developed on the basis of an aptamer that was obtained via Capture-SELEX and by using an ISFET. This biosensor can be used for vanillin detection in presence of interferents and in real sample using an approach of ssDNA probe dehybridization.

Keywords

Vanillin Capture-SELEX Aptamer, ion-selective field-effect transistor Aptasensor, small target, low-weight target 

Notes

Acknowledgements

This work was supported by the Russian Science Foundation (project #16-19-10697).

Data availability

The data that support the findings of this study are available from https://ndownloader.figshare.com/files/9605290.  https://doi.org/10.6084/m9.figshare.5545492.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2017_2586_MOESM1_ESM.docx (1.2 mb)
ESM 1 (DOCX 1239 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Scientific-Manufacturing Complex Technological CenterMoscowRussia

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