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Microwave-assisted synthesis of graphene modified CuO nanoparticles for voltammetric enzyme-free sensing of glucose at biological pH values

Microchimica Acta volume 185, Article number: 57 (2018) Cite this article

Abstract

The effect of graphene nanosheets on the glucose sensing performance of CuO powders was investigated. CuO and graphene-modified CuO nanoparticles (NPs) were fabricated by microwave-assisted synthesis and characterized by X-ray diffraction, transmission electron microscopy and X-ray photoelectron spectroscopy. The material was placed on a glassy carbon electrode (GCE) which then was characterized by cyclic voltammetry and chronoamperometry with respect to the capability of sensing glucose both at pH 13 and pH 7.4. The results revealed that the modified GCE has a fast and selective linear response to glucose at pH 13 that covers the 0.21 μM to 12 mM concentration range, with a 0.21 μM low detection limit. The presence of graphene nanosheets results in an improved sensitivity which is to 700 μA mM−1 cm−2. In solution of pH 7.4, the respective data are a linear analytical range from 5 to 14 mM; a 5 μM LOD and a sensitivity of 37.63 μA mM−1 cm−2 at working potential of −0.05 V (vs. Ag/AgCl) and scan rate of 50 mV s−1. Ascorbic acid, dopamine, uric acid, sucrose, maltose and fructose do not interfere.

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Acknowledgements

Authors gratefully appreciate Shiraz University for the financial support of this research work. M.R. also wishes to thank Daegu Gyeongbuk Institute of Science & Technology (DIGIST) for research support in the form of research collaboration.

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Authors and Affiliations

  1. Department of Materials Science and Engineering, School of Engineering, Shiraz University, Zand Boulevard, Shiraz, 7134851154, Iran

    Faranak Foroughi, Mansour Rahsepar & Mohammad Jafar Hadianfard

  2. Department of Chemistry, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul, 151-747, Republic of Korea

    Hasuck Kim

  3. Department of Energy Systems Engineering, Daegu Gyeongbuk Institute of Science & Technology, Daegu, 711-873, Republic of Korea

    Hasuck Kim

Authors
  1. Faranak Foroughi
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  2. Mansour Rahsepar
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  3. Mohammad Jafar Hadianfard
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  4. Hasuck Kim
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Correspondence to Mansour Rahsepar or Hasuck Kim.

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Foroughi, F., Rahsepar, M., Hadianfard, M.J. et al. Microwave-assisted synthesis of graphene modified CuO nanoparticles for voltammetric enzyme-free sensing of glucose at biological pH values. Microchim Acta 185, 57 (2018). https://doi.org/10.1007/s00604-017-2558-8

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  • DOI: https://doi.org/10.1007/s00604-017-2558-8

Keywords

  • Electrochemical detection
  • Selective glucose detection, phosphate buffer
  • Cyclic voltammetry
  • Chronoamperometry
  • Metal oxide
  • Alkaline pH
  • Human blood serum
  • X ray photoelectron spectroscopy
  • Carbon nanomaterial