Microchimica Acta

, Volume 184, Issue 11, pp 4393–4400 | Cite as

Electrochemical glycoprotein aptasensors based on the in-situ aggregation of silver nanoparticles induced by 4-mercaptophenylboronic acid

  • Ning Xia
  • Cheng Cheng
  • Lin Liu
  • Peizhen Peng
  • Chaoyang Liu
  • Junxue Chen
Original Paper
  • 220 Downloads

Abstract

The authors report on an amperometric method for the determination of glycoprotein by using an aptamer as the bioreceptor. The detection scheme is making use of aggregated citrate-capped silver nanoparticles (AgNPs) on a gold electrode. Aggregation is accomplished by addition of 4-mercaptophenylboronic acid (MPBA) which acts as a cross-linker due to the formation of Ag-S bonds and of boronate esters. A thiolated DNA aptamer was then attached to the electrode in order to capture glycoprotein. Once captured, the glycoprotein reacts with MPBA through the formation of boronate esters. The electrochemical signal is thus amplified by the formation of a network of AgNPs which act as redox reporters. To demonstrate the feasibility of the method, prostate specific antigen (PSA) was chosen as a model analyte. The detection limit for PSA is as low as 0.2 pg mL−1. In our preception, this method provides a powerful tool for studying the glycan function in biological and physiological processes.

Graphical abstract

Schematic of the electrochemical method for the detection of glycoprotein. It is based on 4-mercaptophenylboronic acid (MPBA)-induced in situ formation of citrate-capped silver nanoparticle (AgNP) aggregates as the redox reporters. The MPBA molecules act as the cross-linkers of AgNP assembly based on the formation of Ag-S bonds and on boronate ester covalent interactions.

Keywords

Electrochemical biosensor Glycoprotein Silver nanoparticles Boronic acid Prostate specific antigen Aptamer Signal amplification Boronate esters 

Notes

Acknowledgments

We acknowledge financial support of the National Natural Science Foundation of China (21205003, 21305004), the Joint Fund for Fostering Talents of National Natural Science Foundation of China and Henan Province (U1304205), the Program for Science and Technology Innovation Talents at the University of Henan Province (18HASTIT005) and the Science & Technology Foundation of Henan Province (17A150001).

Compliance with ethical standards

The authors declare that they have no competing interests.

Supplementary material

604_2017_2488_MOESM1_ESM.doc (3 mb)
ESM 1(DOC 3053 kb)

References

  1. 1.
    Ludwig JA, Weinstein JN (2005) Biomarkers in cancer staging, prognosis and treatment selection. Nat Rev Cancer 5:845–856CrossRefGoogle Scholar
  2. 2.
    Pihíková D, Kasák P, Tkac J (2015) Glycoprofiling of cancer biomarkers: label-free electrochemical lectin-based biosensors. Open Chem 13:636–655CrossRefGoogle Scholar
  3. 3.
    Ang SH, Thevarajah M, Alias Y, Khor S (2015) Current aspects in hemoglobin A1c detection: a review. Clin Chim Acta 439:202–211CrossRefGoogle Scholar
  4. 4.
    Zhang Y, Zhuang Y, Shen H, Chen X, Wang J (2017) A super hydrophilic silsesquioxane-based composite for highly selective adsorption of glycoproteins. Microchim Acta 184:1037–1044CrossRefGoogle Scholar
  5. 5.
    Akiba U, Anzai J (2016) Recent progress in electrochemical biosensors for glycoproteins. Sensors 16:2045CrossRefGoogle Scholar
  6. 6.
    Pihikova D, Kasak P, Kubanikova P, Sokol R, Tkac J (2016) Aberrant sialylation of a prostate-specific antigen: electrochemical label-free glycoprofiling in prostate cancer serum samples. Anal Chim Acta 934:72–79CrossRefGoogle Scholar
  7. 7.
    Shah AK, Hill MM, Shiddiky MJA, Trau M (2014) Electrochemical detection of glycan and protein epitopes of glycoproteins in serum. Analyst 139:5970–5976CrossRefGoogle Scholar
  8. 8.
    Zhang JJ, Cheng FF, Zheng TT, Zhu JJ (2017) Versatile aptasensor for electrochemical quantification of cell surface glycan and naked-eye tracking glycolytic inhibition in living cells. Biosens Bioelectron 89:937–945CrossRefGoogle Scholar
  9. 9.
    Dosekova E, Filip J, Bertok T, Both P, Kasak P, Tkac J (2016) Nanotechnology in glycomics: applications in diagnostics, therapy, imaging, and separation processes. Med Res Rev 37:514–626CrossRefGoogle Scholar
  10. 10.
    Wang J (2016) Electrochemical biosensing based on noble metal nanoparticles. Microchim Acta 183:1479–1486CrossRefGoogle Scholar
  11. 11.
    Shan J, Ma Z (2017) A review on amperometric immunoassays for tumor markers based on the use of hybrid materials consisting of conducting polymers and noble metal nanomaterials. Microchim Acta 184:969–979CrossRefGoogle Scholar
  12. 12.
    Xia N, Liu L, Chang Y, Hao Y, Wang X (2017) 4-Mercaptophenylboronic acid-induced in situ formation of silver nanoparticle aggregates as labels on an electrode surface. Electrochem Commun 74:28–32CrossRefGoogle Scholar
  13. 13.
    Xia N, Wang X, Zhou B, Wu Y, Mao W, Liu L (2016) Electrochemical detection of amyloid-β oligomers based on the signal amplification of a network of silver nanoparticles. ACS Appl Mater Interfaces 8:19303–19311CrossRefGoogle Scholar
  14. 14.
    Xu JJ, Zhao WW, Song SP, Fan CH, Chen HY (2014) Functional nanoprobes for ultrasensitive detection of biomolecules: an update. Chem Soc Rev 43:1601–1611CrossRefGoogle Scholar
  15. 15.
    Song W, Li H, Liang H, Qiang W, Xu D (2014) Disposable electrochemical aptasensor array by using in situ DNA hybridization inducing silver nanoparticles aggregate for signal amplification. Anal Chem 86:2775–2783CrossRefGoogle Scholar
  16. 16.
    Wei T, Dong T, Wang Z, Bao J, Tu W, Dai Z (2015) Aggregation of individual sensing units for signal accumulation: conversion of liquid-phase colorimetric assay into enhanced surface-tethered electrochemical analysis. J Am Chem Soc 137:8880–8883CrossRefGoogle Scholar
  17. 17.
    Yang YC, Tseng WL (2016) 1,4-Benzenediboronic-acid-induced aggregation of gold nanoparticles: application to hydrogen peroxide detection and biotin-avidin-mediated immunoassay with naked-eye detection. Anal Chem 88:5355–5362CrossRefGoogle Scholar
  18. 18.
    Anzai J (2016) Recent progress in electrochemical biosensors based on phenylboronic acid and derivatives. Mater Sci Eng C 67:737–746CrossRefGoogle Scholar
  19. 19.
    Li M, Zhu W, Marken F, James TD (2015) Electrochemical sensing using boronic acids. Chem Commun 51:14562–14573CrossRefGoogle Scholar
  20. 20.
    Tan L, Chen K, Huang C, Peng R, Luo X, Yang R, Cheng Y, Tang Y (2015) A fluorescent turn-on detection scheme for α-fetoprotein using quantum dots placed in a boronate-modified molecularly imprinted polymer with high affinity for glycoproteins. Microchim Acta 182:2615–2622CrossRefGoogle Scholar
  21. 21.
    Ma Y, Gao Q, Yang XR (2005) Immobilization of glycosylated enzymes on carbon electrodes, and its application in biosensors. Microchim Acta 150:21–26CrossRefGoogle Scholar
  22. 22.
    Jolly P, Formisano N, Tkáč J, Kasák P, Frost CG, Estrela P (2015) Label-free impedimetric aptasensor with antifouling surfacechemistry: A prostate specific antigen case study. Sensors Actuators B Chem 209:306–312CrossRefGoogle Scholar
  23. 23.
    Shen J, Li Y, Gu H, Xia F, Zuo X (2014) Recent development of sandwich assay based on the nanobiotechnologies for proteins, nucleic acids, small molecules, and ions. Chem Rev 114:7631–7677CrossRefGoogle Scholar
  24. 24.
    Liu B, Lu LS, Hua EH, Jiang ST, Xie GM (2012) Detection of the human prostate-specific antigen using an aptasensor with gold nanoparticles encapsulated by graphitized mesoporous carbon. Microchim Acta 178:163–170CrossRefGoogle Scholar
  25. 25.
    Salimi A, Kavosi B, Fathi F, Hallaj R (2013) Highly sensitive immunosensing of prostate-specific antigen based on ionic liquidecarbon nanotubes modified electrode: application as cancer biomarker for prostatebiopsies. Biosens Bioelectron 42:439–446CrossRefGoogle Scholar
  26. 26.
    Yang J, Wen W, Zhang X, Wang S (2015) Electrochemical immunosensor for the prostate specific antigen detection based on carbon nanotube and gold nanoparticle amplification strategy. Microchim Acta 182:1855–1861CrossRefGoogle Scholar
  27. 27.
    Wang Y, Qu Y, Liu G, Hou X, Huang Y, Wu W, Wu K, Li C (2015) Electrochemical immunoassay for the prostate specific antigen using a reduced graphene oxide functionalized with a high molecular-weight silk peptide. Microchim Acta 182:2061–2067CrossRefGoogle Scholar
  28. 28.
    Zhao J, Guo Z, Feng D, Guo J, Wang J, Zhang Y (2015) Simultaneous electrochemical immunosensing of alpha-fetoprotein and prostate specific antigen using a glassy carbon electrode modified with gold nanoparticle-coated silica nanospheres and decorated with azure a or ferrocenecarboxylic acid. Microchim Acta 182:2435–2442CrossRefGoogle Scholar
  29. 29.
    Rahi A, Sattarahmady N, Heli H (2016) Label-free electrochemical aptasensing of the human prostate-specific antigen using gold nanospears. Talanta 156-157:218–224CrossRefGoogle Scholar
  30. 30.
    Zhu Y, Wang H, Wang L, Zhu J, Jiang W (2016) Cascade signal amplification based on copper nanoparticle-reported rolling circle amplification for ultrasensitive electrochemical detection of the prostate cancer biomarker. ACS Appl Mater Interfaces 8:2573–2581CrossRefGoogle Scholar
  31. 31.
    Jiao L, Mu Z, Miao L, Du W, Wei Q, Li H (2017) Enhanced amperometric immunoassay for the prostate specific antigen using Pt-cu hierarchical trigonal bipyramid nanoframes as a label. Microchim Acta 184:423–429CrossRefGoogle Scholar
  32. 32.
    Li Y, Han J, Chen R, Ren X, Wei Q (2015) Label electrochemical immunosensor for prostate-specific antigen based on graphene and silver hybridized mesoporous silica. Anal Biochem 469:76–82CrossRefGoogle Scholar
  33. 33.
    Yang Z, Kasprzyk-Hordern B, Goggins S, Frost CG, Estrela P (2015) A novel immobilization strategy for electrochemical detection of cancer biomarkers: DNA-directed immobilization of aptamer sensors for sensitive detection of prostate specific antigens. Analyst 140:2628–2633CrossRefGoogle Scholar
  34. 34.
    Jolly P, Tamboli V, Harniman RL, Estrela P, Allender CJ, Bowen JL (2016) Aptamer–MIP hybrid receptor for highly sensitive electrochemical detection of prostate specific antigen. Biosens Bioelectron 75:188–195CrossRefGoogle Scholar
  35. 35.
    Qu B, Guo L, Chu X, Wu D-H, Shen G-L, Yu R-Q (2010) An electrochemical immunosensor based on enzyme-encapsulated liposomes and biocatalytic metal deposition. Anal Chim Acta 663:147–152CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria 2017

Authors and Affiliations

  • Ning Xia
    • 1
  • Cheng Cheng
    • 1
  • Lin Liu
    • 1
    • 2
  • Peizhen Peng
    • 1
  • Chaoyang Liu
    • 1
  • Junxue Chen
    • 1
  1. 1.Henan Provincial Key Laboratory of Early Diagnosis and Drug Development for Esophageal CancerAnyang Normal UniversityAnyangPeople’s Republic of China
  2. 2.Henan Key Laboratory of Biomolecular Recognition and SensingShangqiu Normal UniversityShangqiuPeople’s Republic of China

Personalised recommendations