Abstract
Magnetic molecularly imprinted nanoparticles (MMIPs) with improved dispersity and an increased number of adsorption sites are described. Uniform silica layers were first deposited on the surface of Fe3O4 nanoparticles (Fe3O4 NPs) in order to improve the dispersity of magnetic nanoparticles. Then, 4-formylphenylboronic acid (FPBA) as functional monomer was immobilized on the magnetic carriers to improve the efficiency of template eluting and rebinding. A thin layer of polyaniline imprinted with horseradish peroxidase (HRP) as a model glycoprotein was then placed on the magnetic nanoparticles to enhance the dispersity of the resultant MMIPs. These exhibit high adsorption capacity (62 mg g−1), a satisfactory imprinting factor ( 3.78) and short adsorption equilibrium time (40 min) toward HRP, and the limit of detection is 18.7 μg L−1. This kind of MMIPs, therefore, is deemed being a useful tool for extracting low-abundance glycoproteins from even complex samples.
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01 August 2017
An erratum to this article has been published.
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Acknowledgements
Financial supports from the National Natural Science Foundation of China (No. 21475142, 21611140105), CAS President’s International Fellowship Initiative (SL: 191), the funds for Distinguished Young Scientists of Gansu (1506RJDA281) and the top priority program of “One-Three-Five” Strategic Planning of Chinese Academy of Sciences are gratefully acknowledged.
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An erratum to this article is available at https://doi.org/10.1007/s00604-017-2431-9.
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Sun, XY., Ma, RT., Chen, J. et al. Boronate-affinity based magnetic molecularly imprinted nanoparticles for the efficient extraction of the model glycoprotein horseradish peroxidase. Microchim Acta 184, 3729–3737 (2017). https://doi.org/10.1007/s00604-017-2373-2
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DOI: https://doi.org/10.1007/s00604-017-2373-2