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Graphene oxide wrapped Fe3O4@Au nanostructures as substrates for aptamer-based detection of Vibrio parahaemolyticus by surface-enhanced Raman spectroscopy

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Abstract

The authors describe a sensitive surface-enhanced Raman scattering (SERS)-based aptasensor for the detection of the food pathogen Vibrio parahaemolyticus. Nanostructures consisting of Fe3O4@Au particles wrapped with graphene oxide (GO) were used both as SERS substrates and separation tools. A first aptamer (apt 1) was immobilized on the Fe3O4@Au/GO nanostructures to act as a capture probe via the affinity binding of aptamer and V. parahaemolyticus. A second aptamer (apt-2) was modified with the Raman reporter molecule TAMRA to act as a SERS sensing probes that binds to the target the same way as the Fe3O4@Au/GO-apt 1. The sandwich formed between Fe3O4@Au/GO-apt 1/V. parahaemolyticus and apt 2-TAMRA can be separated with the aid of a magnet. The concentration of V. parahaemolyticus can be quantified by measurement of the SERS intensity of TAMRA. Under optimal conditions, the signal is linearly related to the V. parahaemolyticus concentration in the range between 1.4 × 102 to 1.4 × 106 cfu·mL−1, with a detection limit of 14 cfu·mL−1. Recoveries ranging from 98.5% to 105% are found when analyzing spiked salmon samples. In our perception, the assay described here is a useful tool for quantitation of V. parahaemolyticus in real samples.

GO wrapped Fe3O4@Au nanostructures were synthesized as the substrate and modified with with a first aptamer (apt 1) to capture V. parahaemolyticus. TAMRA labelled aptamer 2 was then used as signal probe. The V. parahaemolyticus concentrations are closely related to the Raman intensity of TAMRA.

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Acknowledgements

This work was partly supported by the natural science foundation of Jiangsu Province BK20140155, Key Research and Development Program of Jiangsu Province BE2016306, the National Natural Science Fund of China (NSFC 31401575, 31401576), China Postdoctoral Science Foundation (2016 T90430), and Collaborative innovation center of food safety and quality control in Jiangsu Province.

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Nuo Duan, Mofei Shen, Shijia Wu, Changxin Zhao, Xiaoyuan Ma & Zhouping Wang

  2. School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, China

    Shijia Wu

  3. National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116034, China

    Zhouping Wang

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  1. Nuo Duan
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  2. Mofei Shen
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  3. Shijia Wu
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  4. Changxin Zhao
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  5. Xiaoyuan Ma
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  6. Zhouping Wang
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Correspondence to Shijia Wu or Zhouping Wang.

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Duan, N., Shen, M., Wu, S. et al. Graphene oxide wrapped Fe3O4@Au nanostructures as substrates for aptamer-based detection of Vibrio parahaemolyticus by surface-enhanced Raman spectroscopy. Microchim Acta 184, 2653–2660 (2017). https://doi.org/10.1007/s00604-017-2298-9

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  • DOI: https://doi.org/10.1007/s00604-017-2298-9

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