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A molecularly imprinted polymer based on multiwalled carbon nanotubes for separation and spectrophotometric determination of L-cysteine

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Abstract

The authors describe a molecularly imprinted polymer (MIP) deposited on multiwalled carbon nanotubes (MIP/MWCNTs) for separation and preconcentration of L-cysteine (L-Cys). The MIP was characterized by scanning electron microscopy, X-ray diffraction and FT-IR and via adsorption kinetics and adsorption isotherms. The MIP is shown to be a viable sorbent for L-Cys which subsequently is quantified by spectrophotometry through formation of a charge transfer complex with the DDQ reagent. The experimental parameters affecting separation efficiency and spectrophotometric determination were optimized. Under optimum conditions and at an analytical wavelength of 478 nm, the calibration plot is linear in the 4.0 to 180 ng mL−1 concentration range, and the limit of detection (at an S/N ratio of 3) is 2.3 ng mL−1. The intra-day and inter-day precision are in the range from 2.4 to 3.6%. The method was successfully applied to determination of L-Cys in spiked human serum and water samples where it gave recoveries ranging from 96.6 to 102.4%.

Schematic of the preparation of a  molecularly imprinted polymer coated on the multiwalled carbon nanotube (MIP/MWCNT) by functionalization of MCNTs with methacrylic acid and subsequent polymerization. The MIP/MWCNTs were successfully applied for extraction and spectrophotometric determination of L-Cys by charge transfer (CT) complexation.

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Authors and Affiliations

  1. Faculty of Chemistry, Bu-Ali Sina University, Hamedan, Iran

    Mahdi Hashemi, Zahra Nazari & Davood Bigdelifam

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  1. Mahdi Hashemi
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  2. Zahra Nazari
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  3. Davood Bigdelifam
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Correspondence to Mahdi Hashemi.

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Hashemi, M., Nazari, Z. & Bigdelifam, D. A molecularly imprinted polymer based on multiwalled carbon nanotubes for separation and spectrophotometric determination of L-cysteine. Microchim Acta 184, 2523–2532 (2017). https://doi.org/10.1007/s00604-017-2236-x

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  • DOI: https://doi.org/10.1007/s00604-017-2236-x

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