Abstract
The authors report an aptaelectrode based on graphene modified iron-oxide chitosan hybrid (CHIT-IO-GR) nanocomposite film deposited on fluorine tin oxide (FTO) for the detection of the Mycobacterium tuberculosis specific antigen MPT64. The biotinylated DNA aptamer sequence specific to the MPT64 was immobilized onto the CHIT-IO-GR/FTO electrode by using streptavidin-biotin interactions. XRD, FT-IR, FE-SEM and electrochemical studies were applied to monitor the steps of the fabrication. The aptaelectrode, operated best at typical voltage of 0.44 V, exhibited a limit of detection (LOD) of 0.9 fg⋅mL−1 within 20 min. The biosensor retained about 80% of its initial activity after 10 uses. The potential application of the aptasensor was established by spike-in studies to obtain recoveries between 83 and 95%.
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Acknowledgements
This work has been supported financially by DBT Project BT/PR5503/MED/29/642/2012, UGC-Start UP Grant F.20-1/2012(BSR/20-7(12/2012), and UGC-SAP F.4-7/2015/DRS-111 (SAP-11). Promotion of University Research and Scientific Excellence Programme of Department of Science and Technology (DST-Purse II) is also acknowledged for the financial support. Professor Jagdeep Kaur, Department of Biotechnology, Panjab University is also acknowledged for providing M. leprae protein to conduct interference studies. We extend our thanks to the Central Instrumentation Facility of the University to assist the characterization studies carried out during the research work.
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Thakur, H., Kaur, N., Sabherwal, P. et al. Aptamer based voltammetric biosensor for the detection of Mycobacterium tuberculosis antigen MPT64. Microchim Acta 184, 1915–1922 (2017). https://doi.org/10.1007/s00604-017-2174-7
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DOI: https://doi.org/10.1007/s00604-017-2174-7