Abstract
Three peptides (each containing 13–18 amino acids) were synthesized and used as templates for molecular imprinting and epitope recognition of the Regenerating Protein 1B (REG1B), which is one of the urinary biomarkers for pancreatic ductal adenocarcinoma (PDAC). Poly(ethylene-co-vinyl alcohol)s were employed as the host for molecular imprinting of the peptides. Following their preparation, the molecularly imprinted polymers (MIP) were examined by cyclic voltammetry. The electrochemical responses of a screen-printed gold substrate coated with the MIP were measured at a working voltage of 300 mV (vs. Ag/AgCl); the entire protein and the peptides gave similar responses at concentrations of <1.0 pg⋅mL−1, with detection limits as low as 0.1 pg⋅mL−1. Urine samples from healthy and PDAC patients were then analyzed by using this modified gold electrode, and the results are in agreement with data obtained with ELISA.
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Acknowledgements
We appreciate financial supports from Ministry of Science and Technology of ROC under Contract nos. MOST 104-2220-E-390-001, MOST 104-2220-E-006-006, MOST 105-2918-I-214-001 and MOST105-2221-E-214-036-.
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The author(s) declare that they have no competing interests. The collection of urine samples was approved by the London Brent Research Ethics Committee (REC number 05/Q0408/65).
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Lee, MH., Thomas, J.L., Liao, CL. et al. Polymers imprinted with three REG1B peptides for electrochemical determination of Regenerating Protein 1B, a urinary biomarker for pancreatic ductal adenocarcinoma. Microchim Acta 184, 1773–1780 (2017). https://doi.org/10.1007/s00604-017-2169-4
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DOI: https://doi.org/10.1007/s00604-017-2169-4