Microchimica Acta

, Volume 184, Issue 5, pp 1539–1545 | Cite as

A magnetic relaxation switch aptasensor for the rapid detection of Pseudomonas aeruginosa using superparamagnetic nanoparticles

  • Fei Jia
  • Lei Xu
  • Wenjie Yan
  • Wei Wu
  • Qianqian Yu
  • Xiaojing Tian
  • Ruitong Dai
  • Xingmin Li
Original Paper
  • 266 Downloads

Abstract

The authors describe a rapid and sensitive magnetic relaxation switch (MRSw) aptasensor for the determination of the food-borne pathogen Pseudomonas aeruginosa. An aptamer against P. aeruginosa is covalently bound to superparamagnetic iron oxide nanoparticles. On incubation with P. aeruginosa bacteria, they will be captured by the aptamer, and this affects the formation of SPIO aggregates. The resulting strong increase in the spin-spin relaxation time (T2) is utilized as the analytical information to quantify the bacteria. Under optimized conditions, the assay has a linear range that extends from 100 cfu⋅mL−1 to 106 cfu⋅mL−1, and a detection limit of 50 cfu⋅mL−1 (at an S/N ratio of 3). The method was applied to the detection of P. aeruginosa in (spiked) real food and drinking water samples and was validated by the established plate counting method. This aptasensor is considered to represent a promising platform for the determination of P. aeruginosa. Conceivably, the method may be extended to other food-borne bacteria or biomolecules for which respective aptamers are available.

Graphical abstract

Schematic of the magnetic relaxation switch aptasensor for Pseudomonas aeruginosa detection. The superparamagnetic nanoparticles act as a “switch” to detect aggregated and dispersed states with and without target, which leads to substantial changes in the T2 relaxation time.

Keywords

Transverse relaxation time Carr-Purcell-Meiboom-Gill pulse sequence Foodborne pathogen Particle aggregation Low-field NMR Meat spoilage 

Notes

Acknowledgements

This work was supported by the National S&T Support Program of China and the China Agriculture Research System Poultry-related Science and Technology Innovation Team of Peking (CARS-PSTP).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2017_2142_MOESM1_ESM.doc (709 kb)
ESM 1 (DOC 709 kb)

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Copyright information

© Springer-Verlag Wien 2017

Authors and Affiliations

  • Fei Jia
    • 1
  • Lei Xu
    • 1
  • Wenjie Yan
    • 2
  • Wei Wu
    • 1
  • Qianqian Yu
    • 1
  • Xiaojing Tian
    • 1
  • Ruitong Dai
    • 1
  • Xingmin Li
    • 1
  1. 1.College of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijingChina
  2. 2.College of Applied Arts and ScienceBeijing Union UniversityBeijingChina

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