Amperometric aptasensing of chloramphenicol at a glassy carbon electrode modified with a nanocomposite consisting of graphene and silver nanoparticles
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A nanocomposite prepared from reduced graphene oxide (rGO) and silver nanoparticles (AgNPs) is used in an electrochemical aptasensor for the sensitive and selective determination of the antibiotic chloramphenicol (CAP). The nanocomposite was obtained by electrostatic assembly of AgNPs on the surface of polyelectrolyte-functionalized rGO and then used to modify a glassy carbon electrode. The biosensor is then obtained by immobilizing the aptamer against CAP. When incubated with solutions of CAP, the sensor surface is loaded with CAP due to aptamer recognition. The captured CAP can be electrochemically reduced to yield a current that is strongly enhanced as a result of the excellent electrocatalysis property of the graphene/AgNP-nanocomposite. Under optimum conditions, the calibration plot is linear in the 0.01 to 35 μM concentration range, with a 2 nM detection limit (at 3σ). The sensor is reproducible, stable, selective over homologous interferents, and performs excellently when analyzing CAP in milk samples.
KeywordsAptasensor Antibiotics Electrocatalysis Linear sweep voltammetry Milk analysis
This work was financially supported by the National Natural Science Foundation of China (21475033), Natural Science Foundation of Hubei Province of China (2014CFB279), Scientific Research Foundation for the Returned Overseas Chinese Scholars, Ministry of Education of China (2015–49), and Science Foundation for Creative Research Group of HBNU (T201501).
Compliance with ethical standards
The authors declare that they have no competing interests.
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