Microchimica Acta

, Volume 184, Issue 5, pp 1437–1444 | Cite as

Enhanced and tunable oxygen carrier and amperometric sensor based on a glassy carbon electrode assembly of a hemoglobin-chitosan-Fe3O4 composite

Original Paper


The authors have prepared organized assemblies of a hemoglobin-chitosan(CS)@Fe3O4 composite on glassy carbon electrodes (GCEs) via three strategies with the aim of preparing tunable Hb-coated GCEs with good stability and long-term oxygen storage capability. The formation and morphology of the Hb-CS@Fe3O4 composite was characterized by scanning electrochemical microscopy, XRD and UV–vis spectroscopy. It is shown that Hb is fully integrated into the CS@Fe3O4 and can be manipulated by a magnetic field whilst maintaining its biological activity. In the absence of oxygen, a surface-controlled electrode process occurs with an interfacial electron transfer rate (k s) of 2.14 s−1. The modified GCE also has a favorable oxygen storage lifetime (almost 6 h). One Hb-CS@Fe3O4 film on the electrode displays particularly good electrocatalytic reduction activity towards oxygen. The linear range for detection of O2 is 1.2 × 10−7 ~ 2.0 × 10−4 mol L−1 with a detection limit of 4.0 × 10−8 mol L−1. In our opinion, this method has great potential in terms of enhanced oxygen storage capability of Hb, which can be applied in special situations such as space operations, down hole mining, mountaineering and diving.

Graphical Abstract

Hb-CS@Fe3O4 composites were prepared by three strategies, and oxygen carrying capability was studied. The corresponding modified electrode constructed on the basis of the magnetic field environment was superior in terms of stability, sensitivity and O2 storage time, showing wider linear range and lower detection limit.


Hemoglobin Fe3O4 nanoparticles Oxygen storage capability Functional electrode interface Amperometry XRD 



This work was funded by the National Natural Science Foundation of China (21475070, 31400847), the Jiangsu Province Natural Science Foundation (BK20151267) and Foundation of Nantong Natural Science Projects (MS12015030, MS12015028, MS12015046). A project funded by the priority academic program development of Jiangsu Higher Education Institutions (PAAD).

Compliance with ethical standards

We declare that the funds mentioned in article have no competing interests.

Supplementary material

604_2017_2137_MOESM1_ESM.pdf (760 kb)
ESM 1 (PDF 759 kb)


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Copyright information

© Springer-Verlag Wien 2017

Authors and Affiliations

  1. 1.School of Public Health, Center of Analysis and Testing, Institute of Analytical Chemistry for Life ScienceNantong UniversityNantongPeople’s Republic of China
  2. 2.Institute of Analytical ChemistrySoochow UniversitySuzhouPeople’s Republic of China
  3. 3.Suzhou Institute of Biomedical Engineering and TechnologyChinese Academy of SciencesSuzhouPeople’s Republic of China

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