Microchimica Acta

, Volume 184, Issue 5, pp 1517–1527 | Cite as

Thymine chitosan nanomagnets for specific preconcentration of mercury(II) prior to analysis using SELDI-MS

  • Hani Nasser Abdelhamid
  • Yu Chih Lin
  • Hui-Fen Wu
Original Paper


Laser desorption-ionization mass spectrometry (LDI-MS) is used to determine Hg(II) ions by using thymine-modified chitosan-coated magnetic nanoparticles (TCTS). TCTS nanoparticles are characterized using transmission electron microscopy, X-ray diffraction, UV-vis absorption, infrared spectroscopy and LDI-MS. TCTS acts as a preconcentration probe, supports surface enhanced LDI-MS (SELDI-MS) and acts as a capping agent for Hg(II). The separation of Hg(II) via this method combined with SELDI-MS provides a sensitive and selective tool for inexpensive and fast (5 min) detection of Hg(II) with limit of detection down to 0.05 nmol for environmental samples such as tap and sea water.

Graphical abstract

Schematic of laserr desorption/ionization mass spectrometry (LDI-MS) using thymine - modified chitosan magnetic nanoparticles for the specific analysis of Hg(II) ions in tap and sea water. TCTS acts as preconcentration probe, supports surface enhanced LDI-MS, and binds Hg(II) ions.


Mercury Laser desorption ionization mass spectrometry Magnetic nanoparticles Thymine Chitosan 



We acknowledge the financial support from the Ministry of science and technology Taiwan. H.N. Abdelhamid expresses his gratitude to Assuit University and Ministry of Higher Education (Egypt) for support.

Compliance with ethical standards

The authors declare that they have no competing interests.

Supplementary material

604_2017_2125_MOESM1_ESM.docx (778 kb)
ESM 1 (DOCX 778 kb)


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Copyright information

© Springer-Verlag Wien 2017

Authors and Affiliations

  • Hani Nasser Abdelhamid
    • 1
    • 2
  • Yu Chih Lin
    • 1
  • Hui-Fen Wu
    • 1
    • 3
    • 4
    • 5
  1. 1.Department of Chemistry and Center for Nanoscience and NanotechnologyNational Sun Yat-Sen UniversityKaohsiungTaiwan
  2. 2.School of Pharmacy, College of PharmacyKaohsiung Medical UniversityKaohsiungTaiwan
  3. 3.Institute of Medical Science and TechnologyNational Sun Yat-Sen UniversityKaohsiungTaiwan
  4. 4.National Sun Yat-Sen University and Academia SinicaKaohsiungTaiwan
  5. 5.Department of ChemistryAssuit UniversityAssiutEgypt

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