Advertisement

Microchimica Acta

, Volume 184, Issue 5, pp 1335–1343 | Cite as

Determination of tetracycline using imprinted polymethacrylates along with fluorescent CdTe quantum dots on plastic substrates

  • Jian-Lian Chen
Original Paper

Abstract

Molecular imprints (MIPs) composed of CdTe quantum dots and tetracycline-imprinted polymethacrylates were synthesized on the surface of flexible plastic polyimide sheets from methacrylic acid, allyl mercaptan, and ethylene glycol dimethacrylate. The amount of aqueous CdTe solution, ethanol diluent, the reaction time and temperature of the radical-initiated polymerization were optimized. The MIP-QD composites were then copolymerized on the methacrylated polyimides. The resulting films were then immersed into a supersonic water-ethanol bath to strip off the tetracycline (Tc) templates. The fluorescence quenching intensity measured at 565 nm under 315 nm excitation before and after dropping a Tc sample on the film was used to optimize the processes. The calibration plot is linear in the 70 μM to 2.2 mM Tc concentration range at pH 7.5, and the LOD is 8.8 μM. The relative standard deviation is 8.2% (for n = 10). The method was applied to the determination of Tc in spiked samples of bovine serum albumin and fetal bovine serum and gave recoveries of 98% (at 200 μg⋅mL−1) and 97% (at 1.0 ppt), respectively.

Graphical abstract

Flexible polyimide substrates were chemically modified with methacrylate groups for anchoring the composites (MIP-QD) of tetracycline-imprinted polymers and CdTe quantum dots. The fluorescence of the integrated substrates is quenched by tetracycline but not by methacycline and steroids.

Keywords

Allyl mercaptan Bovine serum albumin Derivatization Ethylene glycol dimethacrylate Fetal bovine serum Methacrylic acid Quenching Polyimide 

Notes

Acknowledgements

Support for this work by the Ministry of Science and Technology under Grant no. MOST–105–2119–M–039–001 and the China Medical University under Grant no. CMU104-S-30 is gratefully acknowledged.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2017_2118_MOESM1_ESM.docx (708 kb)
ESM 1 (DOCX 708 kb)

References

  1. 1.
    Uddiun ASMI, Yaqoob U, Phan DT, Chung GS (2016) Novel flexible acetylene gas sensor based on PI/PTFE-supported Ag-loaded vertical ZnO nanorods array. Sensors Actuators B Chem 222:536–543CrossRefGoogle Scholar
  2. 2.
    Ruecha N, Rodthongkum N, Cate DM, Volckens J, Chailapakul O, Henry CS (2015) Sensitive electrochemical sensor using a graphene–polyaniline nanocomposite for simultaneous detection of Zn(II), Cd(II), and Pb(II). Anal Chim Acta 874:40–48CrossRefGoogle Scholar
  3. 3.
    Zhang H, Zhang Y, Lin Y, Liang T, Chen Z, Li J, Yue Z, Lv J, Jiang Q, Yi C (2015) Ultrasensitive detection and rapid identification of multiple foodborne pathogens with the naked eyes. Biosens Bioelectron 71:186–193CrossRefGoogle Scholar
  4. 4.
    Wen J, Shi X, He Y, Zhou J, Li Y (2012) Novel plastic biochips for colorimetric detection of biomolecules. Anal Bioanal Chem 404:1935–1944CrossRefGoogle Scholar
  5. 5.
    Barbosa AI, Gehlot P, Sidapra K, Edwards AD, Reis NM (2015) Portable smart phone quantitation of prostate specific antigen (PSA) in a fluoropolymer microfluidic device. Biosens Bioelectron 70:5–14CrossRefGoogle Scholar
  6. 6.
    Moschallski M, Evers A, Brandstetter T, Rühe J (2013) Sensitivity of microarray based immunoassays using surface-attached hydrogels. Anal Chim Acta 781:72–79CrossRefGoogle Scholar
  7. 7.
    Ibáñez AJ, Schüler T, Möller R, Fritzsche W, Saluz HP, Svatoš A (2008) DNA detection using a triple readout optical/AFM/MALDI planar microwell plastic chip. Anal Chem 80:5892–5898CrossRefGoogle Scholar
  8. 8.
    Yang T, Yu YZ, Zhu LS, Wu X, Wang XH, Zhang J (2015) Fabrication of silver interdigitated electrodes on polyimide films via surface modification and ion-exchange technique and its flexible humidity sensor application. Sensors Actuators B Chem 208:327–333CrossRefGoogle Scholar
  9. 9.
    Hashizume M, Maeda M, Iijima K (2013) Biomimetic calcium phosphate coating on polyimide films by utilizing surface-selective hydrolysis treatments. J Ceram Soc Jpn 121:816–818CrossRefGoogle Scholar
  10. 10.
    Zhang J, Tian G, Qi S, Wu Z, Wu D, Jin R (2012) Fabrication and mechanism study of CuO layers on double surfaces of polyimide substrate using surface modification. Compos Sci Technol 72:1020–1026CrossRefGoogle Scholar
  11. 11.
    Park YJ, Yu DM, Ahn JH, Choi JH, Hong YT (2012) Surface modification of polyimide films by an ethylenediamine treatment for a flexible copper clad laminate. Macromol Res 20:168–173CrossRefGoogle Scholar
  12. 12.
    Cui L, He XP, Chen GR (2015) Recent progress in quantum dot based sensors. RSC Adv 5:26644–26653CrossRefGoogle Scholar
  13. 13.
    Wegner KG, Hildebrandt N (2015) Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors. Chem Soc Rev 44:4792–4834CrossRefGoogle Scholar
  14. 14.
    Zhang X, Zhang J, Liu J, Johansson EMJ (2015) Solution processed flexible and bending durable heterojunction colloidal quantum dot solar cell. Nanoscale 7:11520–11524CrossRefGoogle Scholar
  15. 15.
    Wu W, Li F, Nie C, Wu J, Chen W, Wu C, Guo T (2015) Improved performance of flexible white hybrid light emitting diodes by adjusting quantum dots distribution in polymer matrix. Vacuum 111:1–4CrossRefGoogle Scholar
  16. 16.
    Jo CH, Kim JH, Kim J, Kim J, Oh MS, Kang MS, Kim MG, Kim YH, Ju BK, Park SK (2014) Low-temperature annealed PbS quantum dot films for scalable and flexible ambipolar thin-film transistors and circuits. J. Mater Chem C 2:10305–10311CrossRefGoogle Scholar
  17. 17.
    Chao MR, Hu CW, Chen JL (2016) Fluorometric determination of copper(II) using CdTe quantum dots coated with 1-(2-thiazolylazo)-2-naphthol and an ionic liquid. Microchim Acta 183:1323–1332CrossRefGoogle Scholar
  18. 18.
    Duran GM, Contento AM, Rios A (2015) Beta-cyclodextrin coated CdSe/ZnS quantum dots for vanillin sensoring in food samples. Talanta 131:286–291CrossRefGoogle Scholar
  19. 19.
    Mansur HS, Mansur AAP, Soriano-Araújo A, Lobato ZIP, de Carvalho SM, Leite MF (2015) Water-soluble nanoconjugates of quantum dot-chitosan-antibody for in vitro detection of cancer cells based on “enzyme-free” fluoroimmunoassay. Mater Sci Eng C 52:61–71CrossRefGoogle Scholar
  20. 20.
    Lin B, Yu Y, Li R, Cao Y, Guo M (2016) Turn-on sensor for quantification and imaging of acetamiprid residues based on quantum dots functionalized with aptamer. Sensors Actuators B Chem 229:100–109CrossRefGoogle Scholar
  21. 21.
    Chao MR, Hu CW, Chen JL (2014) Comparative syntheses of tetracycline-imprinted polymeric silicate and acrylate on CdTe quantum dots as fluorescent sensors. Biosens Bioelectron 61:471–477CrossRefGoogle Scholar
  22. 22.
    Chao MR, Hu CW, Chen JL (2014) Fluorescent turn-on detection of cysteine using a molecularly imprinted polyacrylate linked to allylthiol-capped CdTe quantum dots. Microchim Acta 181:1085–1091CrossRefGoogle Scholar
  23. 23.
    Lin CI, Joseph AK, Chang CK, Lee YD (2004) Synthesis and photoluminescence study of molecularly imprinted polymers appended onto CdSe/ZnS core-shells. Biosens Bioelectron 20:127–131CrossRefGoogle Scholar
  24. 24.
    Ge S, Zhang C, Yu F, Yan M, Yu J (2011) Layer-by-layer self-assembly CdTe quantum dots and molecularly imprinted polymers modified chemiluminescence sensor for deltamethrin detection. Sens. Actuators B 156:222–227CrossRefGoogle Scholar
  25. 25.
    Chao MR, Hu CW, Chen JL (2016) Glass substrates crosslinked with tetracycline-imprinted polymeric silicate and CdTe quantum dots as fluorescent sensors. Anal Chim Acta 925:61–69CrossRefGoogle Scholar
  26. 26.
    Odian G (2004) Principles of polymerization, 4th edn. John Wiley & Sons, New JerseyCrossRefGoogle Scholar
  27. 27.
    Gürtler C, Danielmeier K (2004) A catalyst system for the reaction of carboxylic acids with aliphatic isocyanates. Tetrahedron Lett 45:2515–2521CrossRefGoogle Scholar
  28. 28.
    Ruedas-Rama MJ, Hall EAH (2008) A quantum dot–lucigenin probe for Cl. Analyst 133:1556–1566CrossRefGoogle Scholar
  29. 29.
    Cai ZX, Yang H, Zhang Y, Yan XP (2006) Preparation,characterization and evaluation of water-soluble L-cysteine-capped-CdS nanoparticles as fluorescence probe for detection of Hg(II) in aqueous solution. Anal Chim Acta 559:234–239CrossRefGoogle Scholar
  30. 30.
    Li J, Mei F, Li WY, He XW, Zhang YK (2008) Study on the fluorescence resonance energy transfer between CdTe QDs and butyl-rhodamine B in the presence of CTMAB and its application on the detection of Hg(II). Spectrochim Acta A 70:811–817CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Wien 2017

Authors and Affiliations

  1. 1.School of PharmacyChina Medical UniversityTaichungTaiwan

Personalised recommendations