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Electrochemiluminescence immunoassay for the carcinoembryonic antigen using CdSe:Eu nanocrystals

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Abstract

CdSe:Eu nanocrystals were successfully synthesized and characterized by transmission electron microscopy, X-ray powder diffraction, and X-ray photoelectric spectroscopy. The CdSe:Eu nanocrystals showed enhanced green electrochemiluminescence (ECL) intensity when compared to pure CdSe nanocrystals. Further, the nanocrystals were used to design an ECL immunosensor for the detection of carcinoembryonic antigen (CEA) that has a linear response over the 1.0 fg·mL−1 to 100 ng·mL−1 CEA concentration range with a 0.4 fg·mL−1 detection limit. The assay was applied to the determination of CEA in human serum samples.

Schematic of the assay: GCE-glassy-carbon electrode, Ab- Antibody, BSA- Bovine serum albumin, Ag- Antigen. CdSe:Eu nanocrystals were used to design an ECL immunosensor for the detection of carcinoembryonic antigen.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant Nos. 21471001, 21427807, 21575001), Natural Science Foundation of Anhui Province (1508085 MB37), Key Project of Anhui Provincial Education Department (KJ2014A016), and 211 project of Anhui University.

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Anhui University, Hefei, 230039, People’s Republic of China

    Qiao Liu, Xing-Pei Liu, Yu-Pin Wei, Chang-Jie Mao, He-Lin Niu, Ji-Ming Song, Bao-Kang Jin & Sheng-Yi Zhang

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  1. Qiao Liu
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  2. Xing-Pei Liu
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Correspondence to Chang-Jie Mao.

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Liu, Q., Liu, XP., Wei, YP. et al. Electrochemiluminescence immunoassay for the carcinoembryonic antigen using CdSe:Eu nanocrystals. Microchim Acta 184, 1353–1360 (2017). https://doi.org/10.1007/s00604-017-2114-6

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  • DOI: https://doi.org/10.1007/s00604-017-2114-6

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