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Microchimica Acta

, Volume 184, Issue 5, pp 1353–1360 | Cite as

Electrochemiluminescence immunoassay for the carcinoembryonic antigen using CdSe:Eu nanocrystals

  • Qiao Liu
  • Xing-Pei Liu
  • Yu-Pin Wei
  • Chang-Jie Mao
  • He-Lin Niu
  • Ji-Ming Song
  • Bao-Kang Jin
  • Sheng-Yi Zhang
Original Paper

Abstract

CdSe:Eu nanocrystals were successfully synthesized and characterized by transmission electron microscopy, X-ray powder diffraction, and X-ray photoelectric spectroscopy. The CdSe:Eu nanocrystals showed enhanced green electrochemiluminescence (ECL) intensity when compared to pure CdSe nanocrystals. Further, the nanocrystals were used to design an ECL immunosensor for the detection of carcinoembryonic antigen (CEA) that has a linear response over the 1.0 fg·mL−1 to 100 ng·mL−1 CEA concentration range with a 0.4 fg·mL−1 detection limit. The assay was applied to the determination of CEA in human serum samples.

Graphical abstract

Schematic of the assay: GCE-glassy-carbon electrode, Ab- Antibody, BSA- Bovine serum albumin, Ag- Antigen. CdSe:Eu nanocrystals were used to design an ECL immunosensor for the detection of carcinoembryonic antigen.

Keywords

CdSe:Eu nanocrytals Electrochemiluminescence XPS Serum analysis Tumor diagnosis 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant Nos. 21471001, 21427807, 21575001), Natural Science Foundation of Anhui Province (1508085 MB37), Key Project of Anhui Provincial Education Department (KJ2014A016), and 211 project of Anhui University.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2017_2114_MOESM1_ESM.docx (5.1 mb)
ESM 1 (DOCX 5183 kb)

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Copyright information

© Springer-Verlag Wien 2017

Authors and Affiliations

  • Qiao Liu
    • 1
  • Xing-Pei Liu
    • 1
  • Yu-Pin Wei
    • 1
  • Chang-Jie Mao
    • 1
  • He-Lin Niu
    • 1
  • Ji-Ming Song
    • 1
  • Bao-Kang Jin
    • 1
  • Sheng-Yi Zhang
    • 1
  1. 1.School of Chemistry and Chemical EngineeringAnhui UniversityHefeiPeople’s Republic of China

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