In-line monitoring of pH and oxygen during enzymatic reactions in off-the-shelf all-glass microreactors using integrated luminescent microsensors
- 425 Downloads
We describe a maskless photopolymerization technique for integration of luminescent sensors for determination of pH values and dissolved oxygen (DO) into commercially available all-glass microfluidic reactors (in sizes as small as 43 ± 3 μm for both DO and pH sensors). The sensor spots are poly(ethyleneglycol acrylate)-based and obtained by photopolymerization of oligomers in the presence of optical probes for oxygen and pH values, respectively. The resulting devices were applied to in-line monitoring of enzymatic conversions of acetylcholine and glucose (by using acetylcholine esterase and glucose oxidase, respectively) on the microscale. The detection limits are 258 μM and 505 μM for acetylcholine and glucose, respectively. The method has a large potential in terms of monitoring enzymatic and other chemical reactions on the microscale in that it does not require special instrumentation apart from a standard widefield fluorescence microscope.
KeywordsMaskless photopolymerization Commercial micro flow reactor Microfluidic chip pH sensor Oxygen sensor Chemical sensor Integrated sensor Glucose oxidase Acetylcholine esterase Fluorescence microscopy
Financial support of this work by the German Research Foundation (DFG, NA 947/1-2 and 2-1) is gratefully acknowledged.
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 6.Funfak A, Hartung R, Cao J, Martin K, Wiesmüller K-H, Wolfbeis OS, Köhler JM (2009) Highly resolved dose–response functions for drug-modulated bacteria cultivation obtained by fluorometric and photometric flow-through sensing in microsegmented flow. Sensors Actuators B Chem 142:66–72. doi: 10.1016/j.snb.2009.07.017 CrossRefGoogle Scholar
- 25.Poehler E, Herzog C, Lotter C, Pfeiffer SA, Aigner D, Mayr T, Nagl S (2015) Label-free microfluidic free-flow isoelectric focusing, pH gradient sensing and near real-time isoelectric point determination of biomolecules and blood plasma fractions. Analyst 140:7496–7502. doi: 10.1039/C5AN01345C CrossRefGoogle Scholar