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Voltammetric myoglobin sensor based on a glassy carbon electrode modified with a composite film consisting of carbon nanotubes and a molecularly imprinted polymerized ionic liquid

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Abstract

The ionic liquid 1-{3-[(2-aminoethyl)amino]propyl}-3-vinylimidazole bromide was synthesized and used to fabricate a molecularly imprinted film for electrochemical sensing of myoglobin (Myo). This film was deposited on a glassy carbon electrode modified with multi-walled carbon nanotubes by using the ionic liquid as the functional monomer, Myo as the template, N,N′-methylenebisacrylamide as the crosslinker, and a redox system containing ammonium persulfate and N,N,N′,N′-tetramethylethylenediamine as the initiator. The sensing performance of the modified electrode was investigated by using the hexacyanoferrate system as an electrochemical redox probe. The results demonstrated that the sensor possesses good selectivity and high sensitivity. The oxidation peak current at the potential of ~0.3 V (vs. SCE) was found linearly related to the myoglobin concentration in the range from 60.0 nM to 6.0 μM, with a 9.7 nM detection limit at an S/N ratio of 3. The sensor was applied to the determination of Myo in spiked serum samples where it showed average recoveries (for n = 5) of 96.5 %.

By using a polymerizable ionic liquid as the functional monomer, a myoglobin imprinted polymer was fabricated on a multi-walled carbon nanotube modified glassy carbon electrode. The sensing performances of the molecularly imprinted sensor towards myoglobin demonstrated good selectivity, sensitivity and accuracy.

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Acknowledgments

The authors gratefully acknowledge the financial supports from the National Natural Science Foundation of China (No.21275166 and 21675175), the Natural Science Foundation of Hubei Province, China (No. 2015CFA092), China Scholarship Council (No. 201307780006). The first two authors contributed equally to this work.

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Authors and Affiliations

  1. Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan, 430074, China

    Yanying Wang, Miao Han, Xiaoxue Ye & Chunya Li

  2. Key Laboratory for Material Chemistry of Energy Conversion and Storage, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, 430074, China

    Yanying Wang & Kangbing Wu

  3. Department of Chemistry, University of Wisconsin-Platteville, 1 University Plaza, Platteville, WI, 53818-3099, USA

    Tsunghsueh Wu

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  1. Yanying Wang
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  2. Miao Han
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  3. Xiaoxue Ye
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  4. Kangbing Wu
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  5. Tsunghsueh Wu
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  6. Chunya Li
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Correspondence to Chunya Li.

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Wang, Y., Han, M., Ye, X. et al. Voltammetric myoglobin sensor based on a glassy carbon electrode modified with a composite film consisting of carbon nanotubes and a molecularly imprinted polymerized ionic liquid. Microchim Acta 184, 195–202 (2017). https://doi.org/10.1007/s00604-016-2005-2

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  • DOI: https://doi.org/10.1007/s00604-016-2005-2

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