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Chitosan-coated hemoglobin microcapsules for use in an electrochemical sensor and as a carrier for oxygen

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Abstract

The article describes the preparation of chitosan-coated hemoglobin (Hb-CS) microcapsules by (a) preparing a CaCO3 precipitate containing Hb, (b) crosslinking Hb with glutaraldehye, (c) coating the particles with chitosan, and (d) preparing Hb-CS microcapsules by removing the CaCO3 template with a solution of disodium EDTA. The morphology and electrochemical properties of the Hb-CS microcapsules were investigated by scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. An oxygen sensor was obtained by immobilizing the Hb-CS microcapsules on the surface of a glassy carbon electrode (GCE) first modified with gold nanoparticles. The application of Hb-CS microcapsules facilitates electron transfer on the surface of GCE and warrants the integrity and biological activity of Hb. The oxygen sensor, operated best at a working voltage of −0.335  V (vs. SCE), displays a low limit of detection (30 nM). The Hb-CS microcapsules also are shown to release loaded oxygen to an anaerobic aqueous environment within 300 min.

The hemoglobin-chitosan microcapsule shows an excellent ability of electrocatalysis and carrying of oxygen.

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Acknowledgments

The authors would like to thank the National Natural Science Foundation of China (NSFC, Nos. 21075087, 21175097, 81202249 and 21375066) and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions for financial support of this research.

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Authors and Affiliations

  1. The Key Lab of Health Chemistry & Molecular Diagnosis of Soochow, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, Soochow, 215123, China

    Yang Liu, Pei She, Shouming Xu, Jianguo Li & Anping Deng

  2. School of Public Health, Nantong University, Nantong, 226019, China

    Yang Liu, Qi Wang, Jin Gong & Weiping Wu

Authors
  1. Yang Liu
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  2. Qi Wang
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  3. Pei She
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  4. Jin Gong
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  5. Weiping Wu
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  7. Jianguo Li
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  8. Kang Zhao
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  9. Anping Deng
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Correspondence to Jianguo Li or Anping Deng.

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Liu, Y., Wang, Q., She, P. et al. Chitosan-coated hemoglobin microcapsules for use in an electrochemical sensor and as a carrier for oxygen. Microchim Acta 183, 2847–2854 (2016). https://doi.org/10.1007/s00604-016-1908-2

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  • DOI: https://doi.org/10.1007/s00604-016-1908-2

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