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Determination of human serum albumin using a single-walled carbon nanotube-FET modified with bromocresol green

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Abstract

We developed a nanoscale-based assay for the detection of human serum albumin (HSA) by placing the HSA indicator probe, bromocresol green (BCG) on the single-walled carbon nanotubes on a field effect transistor (swCNT-FET) via simple coating. Bovine serum albumin (BSA) was used as a blocking agent to reduce non-specific binding. The selective binding of HSA onto BCG alters the charge of the dye and the surface, and this causes a change in the conductance of the swCNT-FET. The assay is highly sensitive and selective for HSA, with an 18.6 ng⋅mL−1 detection limit. The high sensitivity and selectivity of the assay is ascribed to appropriate BSA blocking and the instantaneous response of sensitive swCNT-FET to rapid BCG-HSA binding, which minimizes non-specific binding and overcomes the time-sensitivity of BCG-HSA binding, respectively.

Highly sensitive and selective determination of human serum albumin (BSA) is demonstrated using a single-walled carbon nanotube-field effect transistor (swCNT-FET) modified with bromocresol green (BCG) and by using bovine serum albumin (BSA) as the blocking agent.

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Acknowledgment

This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2013R1A1A2012434). This work was also supported by the Soonchunhyang University Research Fund.

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  1. Department of Chemistry, Soonchunhyang University, Asan, 31538, South Korea

    Bongsu Kim & Tae Hyun Kim

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  1. Bongsu Kim
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  2. Tae Hyun Kim
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Correspondence to Tae Hyun Kim.

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Kim, B., Kim, T.H. Determination of human serum albumin using a single-walled carbon nanotube-FET modified with bromocresol green. Microchim Acta 183, 1513–1518 (2016). https://doi.org/10.1007/s00604-016-1815-6

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  • DOI: https://doi.org/10.1007/s00604-016-1815-6

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