The electrochemical behavior of adenine at a glassy carbon electrode (GCE) modified with a nanocomposite consisting of graphene oxide and polyaniline was investigated by cyclic voltammetry and differential pulse voltammetry. The nanocomposite was synthesized by polymerization and characterized by Raman and UV-vis spectroscopy, and its morphology was examined by scanning electron microscopy. Adenine is oxidized at the modified GCE at a working potential of 1.2 V (vs. Ag/AgCl) and gives a current density of approximately 2.64 nA.cm−2, which is distinctly increased compared to the 0.57 nA.cm−2 of a bare electrode. Peak current and adenine concentration are linearly related to each other in the range from 0.5 μM to 20 μM. The modified GCE exhibits acceptable analytical performance, with a detection limit of 72 nM and a limit of quantification of 240 nM. It is excellently reproducible, stable, and fabrication is simple.
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Financial support from Postdoc I, No. CZ.1.07/2.3.00/30.0009 and GACR P102/11/1068, NanoBioTeCell, the project CEITEC 2020 (LQ1601) and LH 13053 KONTAKT II of the Ministry of Education, Youth and Sports of the Czech Republic is highly acknowledged.
The author(s) declare that they have no competing interests.
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Sharma, V., Hynek, D., Trnkova, L. et al. Electrochemical determination of adenine using a glassy carbon electrode modified with graphene oxide and polyaniline. Microchim Acta 183, 1299–1306 (2016). https://doi.org/10.1007/s00604-015-1740-0
- Cyclic voltammetry
- Differential pulse voltammetry
- Scanning electron microscopy
- Raman spectroscopy