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Electrochemical determination of adenine using a glassy carbon electrode modified with graphene oxide and polyaniline

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Abstract

The electrochemical behavior of adenine at a glassy carbon electrode (GCE) modified with a nanocomposite consisting of graphene oxide and polyaniline was investigated by cyclic voltammetry and differential pulse voltammetry. The nanocomposite was synthesized by polymerization and characterized by Raman and UV-vis spectroscopy, and its morphology was examined by scanning electron microscopy. Adenine is oxidized at the modified GCE at a working potential of 1.2 V (vs. Ag/AgCl) and gives a current density of approximately 2.64 nA.cm−2, which is distinctly increased compared to the 0.57 nA.cm−2 of a bare electrode. Peak current and adenine concentration are linearly related to each other in the range from 0.5 μM to 20 μM. The modified GCE exhibits acceptable analytical performance, with a detection limit of 72 nM and a limit of quantification of 240 nM. It is excellently reproducible, stable, and fabrication is simple.

The electrochemical behavior of adenine at a glassy carbon electrode (GCE) modified with a graphene oxide (GO) and polyaniline (PANI) was studied. Linear calibration dependence in the range from 0.5 μM to 20 μM was observed, with a detection limit of 72 nM and a limit of quantification of 240 nM.

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Acknowledgments

Financial support from Postdoc I, No. CZ.1.07/2.3.00/30.0009 and GACR P102/11/1068, NanoBioTeCell, the project CEITEC 2020 (LQ1601) and LH 13053 KONTAKT II of the Ministry of Education, Youth and Sports of the Czech Republic is highly acknowledged.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic

    Vimal Sharma & Libuse Trnkova

  2. Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University, Zemedelska 1, 613 00, Brno, Czech Republic

    David Hynek & Rene Kizek

  3. Central European Institute of Technology, Brno University of Technology, Technicka 10, 616 00, Brno, Czech Republic

    David Hynek, Libuse Trnkova, Marian Marik, Rene Kizek & Jaromir Hubalek

  4. Department of Condensed Matter Physics, Faculty of Science, Masaryk University, Kotlarska 2, 611 37, Brno, Czech Republic

    Dusan Hemzal

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  1. Vimal Sharma
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Correspondence to Libuse Trnkova.

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Sharma, V., Hynek, D., Trnkova, L. et al. Electrochemical determination of adenine using a glassy carbon electrode modified with graphene oxide and polyaniline. Microchim Acta 183, 1299–1306 (2016). https://doi.org/10.1007/s00604-015-1740-0

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  • DOI: https://doi.org/10.1007/s00604-015-1740-0

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